2024-03-28T11:04:53Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/144532023-10-09T08:00:10Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Trevijano-Contador, Nuria
author
Roselletti, Elena
author
Garcia-Rodas, Rocio
author
Vecchiarelli, Anna
author
Zaragoza, Oscar
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Comunidad de Madrid (España)
funder
Groupe Lesaffre
2022-05-23T10:29:02Z
2022-05-23T10:29:02Z
2022-02-05
Microorganisms. 2022 Feb 5;10(2):373.
2076-2607
http://hdl.handle.net/20.500.12105/14453
35208830
10.3390/microorganisms10020373
Microorganisms
Cryptococcus neoformans is a pathogenic yeast that can form Titan cells in the lungs, which are fungal cells of abnormally large size. The factors that regulate Titan cell formation in vivo are still unknown, although an increased proportion of these fungal cells of infected mice correlates with induction of Th2-type responses. Here, we focused on the role played by the cytokine IL-17 in the formation of cryptococcal Titan cells using Il17a-/- knockout mice. We found that after 9 days of infection, there was a lower proportion of Titan cells in Il17a-/- mice compared to the fungal cells found in wild-type animals. Dissemination to the brain occurred earlier in Il17a-/- mice, which correlated with the lower proportion of Titan cells in the lungs. Furthermore, knockout-infected mice increased brain size more than WT mice. We also determined the profile of cytokines accumulated in the brain, and we found significant differences between both mouse strains. We found that in Il17a-/-, there was a modest increase in the concentrations of the Th1 cytokine TNF-α. To validate if the increase in this cytokine had any role in cryptococcal morphogenesis, we injected wild-type mice with TNF-α t and observed that fungal cell size was significantly reduced in mice treated with this cytokine. Our results suggest a compensatory production of cytokines in Il17a-/- mice that influences both cryptococcal morphology and dissemination.
eng
C57BL76J
Cryptococcus neoformans
IL17
Il17a−/−
Titan cells
Role of IL-17 in Morphogenesis and Dissemination of Cryptococcus neoformans during Murine Infection
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/14453/1/RoleIL-17MorphogenesisDissemination_2022.pdf
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MD5
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RoleIL-17MorphogenesisDissemination_2022.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/14453/4/RoleIL-17MorphogenesisDissemination_2022.pdf.txt
File
MD5
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text/plain
RoleIL-17MorphogenesisDissemination_2022.pdf.txt