2024-03-29T12:36:42Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/139802023-10-13T06:17:41Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2145com_20.500.12105_2144col_20.500.12105_16980col_20.500.12105_16938col_20.500.12105_2146
Repisalud
author
Blanco-Domínguez, Rafael
author
Sánchez-Díaz, Raquel
author
de la Fuente, Hortensia
author
Jiménez-Borreguero, Luis J
author
Matesanz-Marín, Adela
author
Relaño, Marta
author
Jiménez-Alejandre, Rosa
author
Linillos-Pradillo, Beatriz
author
Tsilingiri, Katerina
author
Martín-Mariscal, María L
author
Alonso-Herranz, Laura
author
Moreno, Guillermo
author
Martín-Asenjo, Roberto
author
García-Guimaraes, Marcos M
author
Bruno, Katelyn A
author
Dauden, Esteban
author
González-Álvaro, Isidoro
author
Villar-Guimerans, Luisa M
author
Martínez-León, Amaia
author
Salvador-Garicano, Ane M
author
Michelhaugh, Sam A
author
Ibrahim, Nasrien E
author
Januzzi, James L
author
Kottwitz, Jan
author
Iliceto, Sabino
author
Plebani, Mario
author
Basso, Cristina
author
Baritussio, Anna
author
Seguso, Mara
author
Marcolongo, Renzo
author
Ricote, Mercedes
author
Fairweather, DeLisa
author
Bueno, Hector
author
Fernández-Friera, Leticia
author
Alfonso, Fernando
author
Caforio, Alida L P
author
Pascual-Figal, Domingo A
author
Heidecker, Bettina
author
Lüscher, Thomas F
author
Das, Saumya
author
Fuster, Valentin
author
Ibáñez, Borja
author
Sánchez-Madrid, Francisco
author
Martin, Pilar
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Comunidad de Madrid (España)
funder
Fundación La Marató TV3
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación BBVA
2022-04-13T11:21:50Z
2022-04-13T11:21:50Z
2021-05-27
N Engl J Med . 2021 May 27;384(21):2014-2027
0028-4793
http://hdl.handle.net/20.500.12105/13980
34042389
10.1056/NEJMoa2003608
1533-4406
The New England journal of medicine
The diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging (which is not universally available). Additional approaches to diagnosis are desirable. We sought to identify a novel microRNA for the diagnosis of acute myocarditis.
To identify a microRNA specific for myocarditis, we performed microRNA microarray analyses and quantitative polymerase-chain-reaction (qPCR) assays in sorted CD4+ T cells and type 17 helper T (Th17) cells after inducing experimental autoimmune myocarditis or myocardial infarction in mice. We also performed qPCR in samples from coxsackievirus-induced myocarditis in mice. We then identified the human homologue for this microRNA and compared its expression in plasma obtained from patients with acute myocarditis with the expression in various controls.
We confirmed that Th17 cells, which are characterized by the production of interleukin-17, are a characteristic feature of myocardial injury in the acute phase of myocarditis. The microRNA mmu-miR-721 was synthesized by Th17 cells and was present in the plasma of mice with acute autoimmune or viral myocarditis but not in those with acute myocardial infarction. The human homologue, designated hsa-miR-Chr8:96, was identified in four independent cohorts of patients with myocarditis. The area under the receiver-operating-characteristic curve for this novel microRNA for distinguishing patients with acute myocarditis from those with myocardial infarction was 0.927 (95% confidence interval, 0.879 to 0.975). The microRNA retained its diagnostic value in models after adjustment for age, sex, ejection fraction, and serum troponin level.
After identifying a novel microRNA in mice and humans with myocarditis, we found that the human homologue (hsa-miR-Chr8:96) could be used to distinguish patients with myocarditis from those with myocardial infarction. (Funded by the Spanish Ministry of Science and Innovation and others.).
eng
A Novel Circulating MicroRNA for the Detection of Acute Myocarditis.
journal article
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URL
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