2024-03-29T13:50:08Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/138672023-11-20T10:06:05Zcom_20.500.12105_2088com_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_2060col_20.500.12105_2089col_20.500.12105_2061
Repisalud
author
Flores-Chavez, Maria
author
Abras, Alba
author
Ballart, Cristina
author
Ibáñez-Pérez, Ismael
author
Perez-Gordillo, Pilar
author
Gállego, Montserrat
author
Muñoz, Carmen
author
Moure, Zaira
author
Sulleiro Igual, Elena
author
Nieto Martinez, Francisco Javier
author
Garcia, Emilia
author
Cruz, Israel
author
Picado, Albert
funder
Foundation for Innovative New Diagnostics (Suiza)
funder
Instituto de Salud Carlos III
funder
Agència de Gestió d'Ajuts Universitaris i de Recerca (AGAUR)
funder
Institució Catalana de Recerca i Estudis Avançats
funder
Barcelona Institute for Global Health
2022-03-28T12:51:20Z
2022-03-28T12:51:20Z
2021-04-20
J Clin Microbiol. 2021 Apr 20;59(5):e01860-20.
http://hdl.handle.net/20.500.12105/13867
33692137
10.1128/JCM.01860-20
1098-660X
Journal of Clinical Microbiology
In Spain, PCR is the tool of choice for the diagnosis of congenital Chagas disease (CD) and serology for diagnosing chronic CD. A loop-mediated isothermal amplification test for Trypanosoma cruzi DNA detection showed good analytical performance and ease of use. We aimed to evaluate the performance of the Loopamp Trypanosoma cruzi detection kit (Eiken Chemical Co. Ltd., Japan) (Tcruzi-LAMP) for congenital and chronic CD diagnosis using well-characterized samples. We included samples from 39 congenital and 174 chronic CD cases and from 48 uninfected children born to infected mothers and 34 nonchagasic individuals. The sensitivity, specificity, and accuracy of Tcruzi-LAMP were estimated using standard case definitions for congenital CD (positive result by parasitological or PCR tests or serology after 9 months of age) and chronic CD (positive serology by at least two tests). The Tcruzi-LAMP results were read by visual examination and a real-time fluorimeter. For congenital CD, Tcruzi-LAMP sensitivity was 97% for both types of reading; specificity was 92% by visual examination and 94% by fluorimeter. For chronic CD, sensitivity was 47% and specificity 100%. The accuracy in congenital CD was >94% versus 56% in chronic CD. The agreement of Tcruzi-LAMP with PCR tests was better in congenital CD (kappa, 0.86 to 0.91) than in chronic CD (kappa, 0.67 to 0.83). The Loopamp Trypanosoma cruzi detection kit showed good performance for the diagnosis of congenital CD. Tcruzi-LAMP, like PCR, can be useful for the screening and early diagnosis of congenital infection.
eng
LAMP
Accuracy
Congenital Chagas disease
Molecular diagnosis
Sensitivity
Specificity
Evaluation of the Performance of the Loopamp Trypanosoma cruzi Detection Kit for the Diagnosis of Chagas Disease in an Area Where It Is Not Endemic, Spain
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/13867/6/EvaluationPerformanceLoopampTrypanosoma_2021.pdf
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https://repisalud.isciii.es/bitstream/20.500.12105/13867/4/EvaluationOfThePerformance_2021.pdf.txt
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EvaluationOfThePerformance_2021.pdf.txt
URL
https://repisalud.isciii.es/bitstream/20.500.12105/13867/7/EvaluationPerformanceLoopampTrypanosoma_2021.pdf.txt
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EvaluationPerformanceLoopampTrypanosoma_2021.pdf.txt