2024-03-29T10:06:59Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/111902024-01-24T21:34:27Zcom_20.500.12105_2152com_20.500.12105_2051com_20.500.12105_2144com_20.500.12105_2145col_20.500.12105_2153col_20.500.12105_2146
Repisalud
author
Rodriguez, Jose Manuel
author
Pozo, Fernando
author
di Domenico, Tomas
author
Vazquez, Jesus
author
Tress, Michael L
funder
National Institutes of Health (Estados Unidos)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación La Marató TV3
funder
Fundación La Caixa
2020-10-21T11:24:16Z
2020-10-21T11:24:16Z
2020-10
PLoS Comput Biol. 2020; 16(10):e1008287
http://hdl.handle.net/20.500.12105/11190
33017396
10.1371/journal.pcbi.1008287
1553-7358
PLoS computational biology
The role of alternative splicing is one of the great unanswered questions in cellular biology. There is strong evidence for alternative splicing at the transcript level, and transcriptomics experiments show that many splice events are tissue specific. It has been suggested that alternative splicing evolved in order to remodel tissue-specific protein-protein networks. Here we investigated the evidence for tissue-specific splicing among splice isoforms detected in a large-scale proteomics analysis. Although the data supporting alternative splicing is limited at the protein level, clear patterns emerged among the small numbers of alternative splice events that we could detect in the proteomics data. More than a third of these splice events were tissue-specific and most were ancient: over 95% of splice events that were tissue-specific in both proteomics and RNAseq analyses evolved prior to the ancestors of lobe-finned fish, at least 400 million years ago. By way of contrast, three in four alternative exons in the human gene set arose in the primate lineage, so our results cannot be extrapolated to the whole genome. Tissue-specific alternative protein forms in the proteomics analysis were particularly abundant in nervous and muscle tissues and their genes had roles related to the cytoskeleton and either the structure of muscle fibres or cell-cell connections. Our results suggest that this conserved tissue-specific alternative splicing may have played a role in the development of the vertebrate brain and heart.
eng
An analysis of tissue-specific alternative splicing at the protein level.
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/11190/1/AnalysisTtissueSpecificAlternative_2020.pdf
File
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AnalysisTtissueSpecificAlternative_2020.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/11190/6/AnalysisTtissueSpecificAlternative_2020.pdf.txt
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AnalysisTtissueSpecificAlternative_2020.pdf.txt