2024-03-29T11:32:10Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/109812023-10-05T09:21:51Zcom_20.500.12105_2174com_20.500.12105_2051com_20.500.12105_2173col_20.500.12105_2175
Repisalud
author
Neupane, Ritam
author
Pisareva, Vera P
author
Rodriguez, Carlos F
author
Pisarev, Andrey V
author
Fernández, Israel S
funder
United States Department of Health and Human Services
2020-09-04T12:35:43Z
2020-09-04T12:35:43Z
2020-04-14
Elife . 2020 ;9:e54575.
http://hdl.handle.net/20.500.12105/10981
32286223
10.7554/eLife.54575
2050-084X
eLife
Taking control of the cellular apparatus for protein production is a requirement for virus progression. To ensure this control, diverse strategies of cellular mimicry and/or ribosome hijacking have evolved. The initiation stage of translation is especially targeted as it involves multiple steps and the engagement of numerous initiation factors. The use of structured RNA sequences, called Internal Ribosomal Entry Sites (IRES), in viral RNAs is a widespread strategy for the exploitation of eukaryotic initiation. Using a combination of electron cryo-microscopy (cryo-EM) and reconstituted translation initiation assays with native components, we characterized how a novel IRES at the 5'-UTR of a viral RNA assembles a functional initiation complex via an uAUG intermediate. The IRES features a novel extended, multi-domain architecture, that circles the 40S head. The structures and accompanying functional data illustrate the importance of 5'-UTR regions in translation regulation and underline the relevance of the untapped diversity of viral IRESs.
eng
EUKARYOTIC TRANSLATION INITIATION
OPEN READING FRAMES
CRYO-EM STRUCTURE
SCANNING MECHANISM
RIBOSOME
VIRUS
PROTEIN
REINITIATION
ELONGATION
REFINEMENT
A complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate.
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/10981/1/AcomplexIRESatthe5%e2%80%99-UTR_2020.pdf
File
MD5
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application/pdf
AcomplexIRESatthe5’-UTR_2020.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/10981/6/AcomplexIRESatthe5%e2%80%99-UTR_2020.pdf.txt
File
MD5
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text/plain
AcomplexIRESatthe5’-UTR_2020.pdf.txt