2024-03-28T11:14:16Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/101082023-10-10T10:09:02Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16985col_20.500.12105_16982col_20.500.12105_2061
Repisalud
author
Bleriot, Ines
author
Trastoy, Rocío
author
Blasco, Lucia
author
Fernández-Cuenca, Felipe
author
Ambroa, Antón
author
Fernández-García, Laura
author
Pacios, Olga
author
Perez-Nadales, Elena
author
Torre-Cisneros, Julian
author
Oteo-Iglesias, Jesus
author
Navarro, Ferran
author
Miró, Elisenda
author
Pascual, Alvaro
author
Bou, German
author
Martínez-Martínez, Luis
author
Tomas, Maria
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Xunta de Galicia (España)
2020-05-14T08:01:25Z
2020-05-14T08:01:25Z
2020-04-29
Microb Genom. 2020 Apr 29.
2057-5858
http://hdl.handle.net/20.500.12105/10108
32375972
10.1099/mgen.0.000369
2057-5858
Microbial genomics
Klebsiella pneumoniae is the clinically most important species within the genus Klebsiella and, as a result of the continuous emergence of multi-drug resistant (MDR) strains, the cause of severe nosocomial infections. The decline in the effectiveness of antibiotic treatments for infections caused by MDR bacteria has generated particular interest in the study of bacteriophages. In this study, we characterized a total of 40 temperate bacteriophages (prophages) with a genome range of 11.454-84.199 kb, predicted from 16 carbapenemase-producing clinical strains of K. pneumoniae belonging to different sequence types, previously identified by multilocus sequence typing. These prophages were grouped into the three families in the order Caudovirales (27 prophages belonging to the family Myoviridae, 10 prophages belonging to the family Siphoviridae and 3 prophages belonging to the family Podoviridae). Genomic comparison of the 40 prophage genomes led to the identification of four prophages isolated from different strains and of genome sizes of around 33.3, 36.1, 39.6 and 42.6 kb. These prophages showed sequence similarities (query cover >90 %, identity >99.9 %) with international Microbe Versus Phage (MVP) (http://mvp.medgenius.info/home) clusters 4762, 4901, 3499 and 4280, respectively. Phylogenetic analysis revealed the evolutionary proximity among the members of the four groups of the most frequently identified prophages in the bacterial genomes studied (33.3, 36.1, 39.6 and 42.6 kb), with bootstrap values of 100 %. This allowed the prophages to be classified into three clusters: A, B and C. Interestingly, these temperate bacteriophages did not infect the highest number of strains as indicated by a host-range assay, these results could be explained by the development of superinfection exclusion mechanisms. In addition, bioinformatic analysis of the 40 identified prophages revealed the presence of 2363 proteins. In total, 59.7 % of the proteins identified had a predicted function, mainly involving viral structure, transcription, replication and regulation (lysogenic/lysis). Interestingly, some proteins had putative functions associated with bacterial virulence (toxin expression and efflux pump regulators), phage defence profiles such as toxin-antitoxin modules, an anti-CRISPR/Cas9 protein, TerB protein (from terZABCDE operon) and methyltransferase proteins.
eng
Klebsiella pneumoniae
Bioinformatics
Comparative genomics
Genomic analysis
Phylogeny
Prophages
Genomic analysis of 40 prophages located in the genomes of 16 carbapenemase-producing clinical strains of Klebsiella pneumoniae
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/10108/1/GenomicAnalysisOf40_2020.pdf
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GenomicAnalysisOf40_2020.pdf
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https://repisalud.isciii.es/bitstream/20.500.12105/10108/5/GenomicAnalysisOf40_2020.pdf.txt
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GenomicAnalysisOf40_2020.pdf.txt