2024-03-28T12:01:03Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/95342022-07-28T12:17:59Zcom_20.500.12105_2102com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2103
00925njm 22002777a 4500
dc
López-Cacho, José Manuel
author
Gallardo, Soledad
author
Posada De la Paz, Manuel
author
Aguerri, Miriam
author
Calzada, David
author
Mayayo, Teodoro
author
González-Rodríguez, María Luisa
author
Rabasco, Antonio María
author
Lahoz, Carlos
author
Cárdaba, Blanca
author
2014
This study aimed to search the correlation among immunological profiles and clinical phenotypes of scleroderma in well-characterized groups of scleroderma patients, comparing forty-nine scleroderma patients stratified according to specific clinical phenotypes with forty-nine healthy controls. Five immunological cell subpopulations (B, CD4(+) and CD8(+) T-cells, NK, and monocytes) and their respective stages of apoptosis and activation were analyzed by flow cytometry, in samples of peripheral blood mononuclear cells (PBMCs). Analyses of results were stratified according to disease stage, time since the diagnosis, and visceral damage (pulmonary fibrosis, pulmonary hypertension, and cardiac affliction) and by time of treatment with corticosteroids. An increase in the percentages of monocytes and a decrease in the B cells were mainly related to the disease progression. A general apoptosis decrease was found in all phenotypes studied, except in localized scleroderma. An increase of B and NK cells activation was found in patients diagnosed more than 10 years ago. Specific cell populations like monocytes, NK, and B cells were associated with the type of affected organ. This study shows how, in a heterogeneous disease, proper patient's stratification according to clinical phenotypes allows finding specific cellular profiles. Our data may lead to improvements in the knowledge of prognosis factors and to aid in the analysis of future specific therapies.
Biomed Res Int. 2014;2014:148293.
2314-6133
http://hdl.handle.net/20.500.12105/9534
24818126
10.1155/2014/148293
2314-6141
BioMed research international
Association of immunological cell profiles with specific clinical phenotypes of scleroderma disease