2024-03-28T18:51:22Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/94732023-07-11T00:00:58Zcom_20.500.12105_2145com_20.500.12105_2051com_20.500.12105_2144col_20.500.12105_2146
00925njm 22002777a 4500
dc
Haider, Nezam
author
Boscá, Lisardo
author
Zandbergen, H Reinier
author
Kovacic, Jason C
author
Narula, Navneet
author
González-Ramos, Silvia
author
Fernandez-Velasco, María
author
Agrawal, Sudhanshu
author
Paz-García, Marta
author
Gupta, Sudhir
author
DeLeon-Pennell, Kristine
author
Fuster, Valentin
author
Ibáñez, Borja
author
Narula, Jagat
author
2019-12-24
BACKGROUND: Macrophages and fibroblasts are 2 major cell types involved in healing after myocardial infarction (MI), contributing to myocardial remodeling and fibrosis. Post-MI fibrosis progression is characterized by a decrease in cardiac macrophage content. OBJECTIVES: This study explores the potential of macrophages to express fibroblast genes and the direct role of these cells in post-MI cardiac fibrosis. METHODS: Prolonged in vitro culture of human macrophages was used to evaluate the capacity to express fibroblast markers. Infiltrating cardiac macrophages was tracked in vivo after experimental MI of LysM(Cre/+);ROSA26(EYFP/+) transgenic mice. The expression of Yellow Fluorescent Protein (YFP) in these animals is restricted to myeloid lineage allowing the identification of macrophage-derived fibroblasts. The expression in YFP-positive cells of fibroblast markers was determined in myocardial tissue sections of hearts from these mice after MI. RESULTS: Expression of the fibroblast markers type I collagen, prolyl-4-hydroxylase, fibroblast specific protein-1, and fibroblast activation protein was evidenced in YFP-positive cells in the heart after MI. The presence of fibroblasts after MI was evaluated in the hearts of animals after depletion of macrophages with clodronate liposomes. This macrophage depletion significantly reduced the number of Mac3+Col1A1+ cells in the heart after MI. CONCLUSIONS: The data provide both in vitro and in vivo evidence for the ability of macrophages to transition and adopt a fibroblast-like phenotype. Therapeutic manipulation of this macrophage-fibroblast transition may hold promise for favorably modulating the fibrotic response after MI and after other cardiovascular pathological processes.
J Am Coll Cardiol. 2019; 74(25):3124-3135
0735-1097
http://hdl.handle.net/20.500.12105/9473
31856969
10.1016/j.jacc.2019.10.036
1558-3597
Journal of the American College of Cardiology
Cardiac fibroblast
Fibroblast markers
Infiltration
Macrophage/fibroblast-like transition
Myeloid tracers
Myocardial infarction
Transition of Macrophages to Fibroblast-Like Cells in Healing Myocardial Infarction