2024-03-28T17:30:24Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/93512023-10-05T08:23:30Zcom_20.500.12105_2174com_20.500.12105_2051com_20.500.12105_2173col_20.500.12105_2175
00925njm 22002777a 4500
dc
Esteva-Socias, Margalida
author
Artiga, Maria Jesus
author
Bahamonde, Olga
author
Belar, Oihana
author
Bermudo, Raquel
author
Castro, Erika
author
Escámez, Teresa
author
Fraga, Máximo
author
Jauregui-Mosquera, Laura
author
Novoa, Isabel
author
Peiró-Chova L
author
Rejón, Juan-David
author
Ruiz-Miró, María
author
Vieiro-Balo, Paula
author
Villar-Campo, Virginia
author
Zazo, Sandra
author
Rábano, Alberto
author
Villena, Cristina
author
2019-11-12
The purpose of the present work is to underline the importance of obtaining a standardized procedure to ensure and evaluate both clinical and research usability of human tissue samples. The study, which was carried out by the Biospecimen Science Working Group of the Spanish Biobank Network, is based on a general overview of the current situation about quality assurance in human tissue biospecimens. It was conducted an exhaustive review of the analytical techniques used to evaluate the quality of human tissue samples over the past 30 years, as well as their reference values if they were published, and classified them according to the biomolecules evaluated: (i) DNA, (ii) RNA, and (iii) soluble or/and fixed proteins for immunochemistry. More than 130 publications released between 1989 and 2019 were analysed, most of them reporting results focused on the analysis of tumour and biopsy samples. A quality assessment proposal with an algorithm has been developed for both frozen tissue samples and formalin-fixed paraffin-embedded (FFPE) samples, according to the expected quality of sample based on the available pre-analytical information and the experience of the participants in the Working Group. The high heterogeneity of human tissue samples and the wide number of pre-analytic factors associated to quality of samples makes it very difficult to harmonize the quality criteria. However, the proposed method to assess human tissue sample integrity and antigenicity will not only help to evaluate whether stored human tissue samples fit for the purpose of biomarker development, but will also allow to perform further studies, such as assessing the impact of different pre-analytical factors on very well characterized samples or evaluating the readjustment of tissue sample collection, processing and storing procedures. By ensuring the quality of the samples used on research, the reproducibility of scientific results will be guaranteed.
J Transl Med. 2019 Nov;17(1):370
1479-5876
http://hdl.handle.net/20.500.12105/9351
31718661
10.1186/s12967-019-2124-8
1479-5876
Journal of translational medicine
Biobank
Biospecimen science
Pre-analytical variables
Quality
Tissue
In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network