2024-03-29T07:25:25Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/89632023-10-05T10:00:31Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
00925njm 22002777a 4500
dc
Gruber, Cesare E M
author
Bartolini, Barbara
author
Castilletti, Concetta
author
Mirazimi, Ali
author
Hewson, Roger
author
Christova, Iva
author
Avšič, Tatjana
author
Grunow, Roland
author
Papa, Anna
author
Sánchez-Seco, María Paz
author
Kopmans, Marion
author
Ippolito, Giuseppe
author
Capobianchi, Maria R
author
Reusken, Chantal B E M
author
Di Caro, Antonino
author
2019
The Crimean-Congo hemorrhagic fever virus (CCHFV) is considered to be a major emerging infectious threat, according to the WHO R&D blueprint. A wide range of CCHFV molecular assays have been developed, employing varied primer/probe combinations. The high genetic variability of CCHFV often hampers the efficacy of available molecular tests and can affect their diagnostic potential. Recently, increasing numbers of complete CCHFV genomic sequences have become available, allowing a better appreciation of the genomic evolution of this virus. We summarized the current knowledge on molecular methods and developed a new bioinformatics tool to evaluate the existing assays for CCHFV detection, with a special focus on strains circulating in different geographical areas. Twenty-two molecular methods and 181 sequences of CCHFV were collected, respectively, from PubMed and GenBank databases. Up to 28 mismatches between primers and probes of each assay and CCHFV strains were detected through in-silico PCR analysis. Combinations of up to three molecular methods markedly decreased the number of mismatches within most geographic areas. These results supported the good practice of CCHFV detection of performing more than one assay, aimed for different sequence targets. The choice of the most appropriate tests must take into account patient's travel history and geographic distribution of the different CCHFV strains.
Viruses. 2019 Oct 16;11(10). pii: E953.
1999-4915
http://hdl.handle.net/20.500.12105/8963
31623214
10.3390/v11100953
1999-4915
Viruses
CCHFV
Crimean–Congo hemorrhagic fever virus
Arthropod-borne virus
Emerging diseases
Laboratory preparedness
Molecular detection
Geographical Variability Affects CCHFV Detection by RT-PCR: A Tool for In-Silico Evaluation of Molecular Assays