2024-03-29T12:48:11Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/88872023-03-24T17:28:45Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16971col_20.500.12105_2061
00925njm 22002777a 4500
dc
Cabrera-Rodríguez, Romina
author
Hebmann, Veronique
author
Marfil, Silvia
author
Pernas, Maria
author
Marrero-Hernández, Sara
author
Cabrera, Cecilia
author
Urrea, Victor
author
Casado, Concepcion
author
Olivares, Isabel
author
Márquez-Arce, Daniel
author
Pérez-Yanes, Silvia
author
Estévez-Herrera, Judith
author
Clotet, Bonaventura
author
Espert, Lucile
author
Lopez-Galindez, Luis Cecilio
author
Biard-Piechaczyk, Martine
author
Valenzuela-Fernández, Agustín
author
Blanco, Julià
author
2019-04-03
In untreated HIV-1-infected individuals, viremia is positively associated with disease progression. However, some viremic non progressors (VNPs) individuals show paradoxical high CD4+ T cell counts. HIV-1 envelope glycoprotein complex (Env) is a major cytopathic determinant in viral replication; therefore, we have deeply characterized Env function in this rare clinical phenotype. Full-length Env clones isolated from individuals with Viral Load (VL) > 10,000 copies/mL classified as VNPs (n = 15) or rapid progressors (RPs, n = 17) were geno- and phenotypically analyzed by determining diversity, expression, CD4 binding/signaling, fusogenicity, infectivity and autophagy induction. Selected Env clones from VNPs and RPs (n = 32) showed similar expression, fusion and infection abilities. Env clones from both groups showed similar affinity for CD4 during cell-to-cell transmission and consistently induced similar levels of CD4 signaling, measured by α-tubulin acetylation. Moreover, we demonstrate for the first time that primary Env clones from VNP and RP induce autophagy in uninfected cells and that this feature correlated with fusogenic capacity but was unrelated to disease progression. In conclusion, our data suggest that Env clones from VNP individuals are fully functional. Therefore, the paradoxical CD4+ T cell count stability coexisting with high levels of viral replication is unrelated to Env function.
Sci Rep. 2019 Apr 3;9(1):5544.
2045-2322
http://hdl.handle.net/20.500.12105/8887
30944395
10.1038/s41598-019-42075-3
2045-2322
Scientific reports
Diabetes mellitus
Índice de masa corporal
Nutrición enteral
Nutrición enteral domiciliaria
Gastrostomía endoscópica percutánea
Antidiabéticos orales
HIV-1 envelope glycoproteins isolated from Viremic Non-Progressor individuals are fully functional and cytopathic