2024-03-28T18:15:05Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/88532022-07-18T09:14:40Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
00925njm 22002777a 4500
dc
Ejazi, Sarfaraz Ahmad
author
Ghosh, Sneha
author
Saha, Samiran
author
Choudhury, Somsubhra Thakur
author
Bhattacharyya, Anirban
author
Chatterjee, Mitali
author
Pandey, Krishna
author
Das, V N R
author
Das, Pradeep
author
Rahaman, Mehebubar
author
Goswami, Rama Prosad
author
Rai, Keshav
author
Khanal, Basudha
author
Bhattarai, Narayan Raj
author
Deepachandi, Bhagya
author
Siriwardana, Yamuna Deepani
author
Karunaweera, Nadira D
author
deBrito, Maria Edileuza Felinto
author
Gomes, Yara de Miranda
author
Nakazawa, Mineo
author
Costa, Carlos Henrique Nery
author
Adem, Emebet
author
Yeshanew, Arega
author
Melkamu, Roma
author
Fikre, Helina
author
Hurissa, Zewdu
author
Diro, Ermias
author
Carrillo, Eugenia
author
Moreno, Javier
author
Ali, Nahid
author
2019-07-09
Visceral leishmaniasis (VL) is one of the leading infectious diseases affecting developing countries. Colloidal gold-based diagnostic tests are rapid tools to detect blood/serum antibodies for VL diagnosis. Lack of uniformity in the performance of these tests in different endemic regions is a hurdle in early disease diagnosis. This study is designed to validate a serum-based dipstick test in eight centres of six countries, India, Nepal, Sri Lanka, Brazil, Ethiopia and Spain with archived and fresh sera from 1003 subjects. The dipstick detects antibodies against Leishmania donovani membrane antigens (LAg). The overall sensitivity and specificity of the test with 95% confidence intervals were found to be 97.10% and 93.44%, respectively. The test showed good sensitivity and specificity in the Indian subcontinent (>95%). In Brazil, Ethiopia, and Spain the sensitivity and specificity of the dipstick test (83.78-100% and 79.06-100%) were better as compared to the earlier reports of the performance of rK39 rapid test in these regions. Interestingly, less cross-reactivity was found with the cutaneous form of the disease in Spain, Brazil, and Sri Lanka demonstrating 91.58% specificity. This dipstick test can therefore be a useful tool for diagnosing VL from other symptomatically similar diseases and against cutaneous form of leishmaniasis.
Sci Rep. 2019 Jul 9;9(1):9932.
2045-2322
http://hdl.handle.net/20.500.12105/8853
31289323
10.1038/s41598-019-46283-9
2045-2322
Scientific reports
A multicentric evaluation of dipstick test for serodiagnosis of visceral leishmaniasis in India, Nepal, Sri Lanka, Brazil, Ethiopia and Spain