2024-03-28T12:03:33Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/83682023-10-09T07:53:20Zcom_20.500.12105_2174com_20.500.12105_2051com_20.500.12105_2173col_20.500.12105_2175
00925njm 22002777a 4500
dc
Segura, Víctor
author
Valero, M Luz
author
Cantero, Laura
author
Muoz Peralta, Javier
author
Zarzuela, Eduardo
author
García, Fernando
author
Aloria, Kerman
author
Beaskoetxea, Javier
author
Arizmendi, Jesús M
author
Navajas, Rosana
author
Paradela, Alberto
author
Díez, Paula
author
Dégano, Rosa Mª
author
Fuentes, Manuel
author
Orfao, Alberto
author
Montero, Andrés García
author
Garin-Muga, Alba
author
Corrales, Fernando J
author
Pino, Manuel M Sánchez Del
author
2018-02-05
Monocytes are bone marrow-derived leukocytes that are part of the innate immune system. Monocytes are divided into three subsets: classical, intermediate and non-classical, which can be differentiated by their expression of some surface antigens, mainly CD14 and CD16. These cells are key players in the inflammation process underlying the mechanism of many diseases. Thus, the molecular characterization of these cells may provide very useful information for understanding their biology in health and disease. We performed a multicentric proteomic study with pure classical and non-classical populations derived from 12 healthy donors. The robust workflow used provided reproducible results among the five participating laboratories. Over 5000 proteins were identified, and about half of them were quantified using a spectral counting approach. The results represent the protein abundance catalogue of pure classical and enriched non-classical blood peripheral monocytes, and could serve as a reference dataset of the healthy population. The functional analysis of the differences between cell subsets supports the consensus roles assigned to human monocytes.
Proteomes. 2018 Feb 5;6(1). pii: E8.
2227-7382
http://hdl.handle.net/20.500.12105/8368
29401756
10.3390/proteomes6010008
Proteomes
monocytes
protein profiling
quantitative proteomics
In-Depth Proteomic Characterization of Classical and Non-Classical Monocyte Subsets