2024-03-29T12:17:39Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/82082023-10-09T14:33:26Zcom_20.500.12105_2174com_20.500.12105_2051com_20.500.12105_2173col_20.500.12105_2175
00925njm 22002777a 4500
dc
Olbrich, Teresa
author
Vega-Sendino, Maria
author
Murga, Matilde
author
de Carcer Diez, Guillermo
author
Malumbres Martinez, Marcos
author
Ortega Jimenez, Sagrario
author
Ruiz, Sergio
author
Fernandez-Capetillo, Oscar
author
2019-07-16
The recent availability of somatic haploid cell lines has provided a unique tool for genetic studies in mammals. However, the percentage of haploid cells rapidly decreases in these cell lines, which we recently showed is due to their overgrowth by diploid cells present in the cultures. Based on this property, we have now performed a phenotypic chemical screen in human haploid HAP1 cells aiming to identify compounds that facilitate the maintenance of haploid cells. Our top hit was 10-Deacetyl-baccatin-III (DAB), a chemical precursor in the synthesis of Taxol, which selects for haploid cells in HAP1 and mouse haploid embryonic stem cultures. Interestingly, DAB also enriches for diploid cells in mixed cultures of diploid and tetraploid cells, including in the colon cancer cell line DLD-1, revealing a general strategy for selecting cells with lower ploidy in mixed populations of mammalian cells.
Cell Rep. 2019;28(3):597-604.
22111247
http://hdl.handle.net/20.500.12105/8208
31315040
10.1016/j.celrep.2019.06.060
2211-1247
Cell reports
10-Deacetylbaccatin-III
Chemical screen
Haploidy
Mitosis
Paclitaxel
Tetraploidy
A Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cells