2024-03-29T05:45:02Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/78772023-10-06T08:13:12Zcom_20.500.12105_2174com_20.500.12105_2051com_20.500.12105_2173col_20.500.12105_2175
00925njm 22002777a 4500
dc
Pérez-Guijarro, Eva
author
Karras, Panagiotis
author
Cifdaloz, Metehan
author
Martínez-Herranz, Raúl
author
Cañón, Estela
author
Graña Castro, Osvaldo
author
Horcajada-Reales, Celia
author
Alonso-Curbelo, Direna
author
Calvo, Tonantzin G
author
Gómez-López, Gonzalo
author
Bellora, Nicolas
author
Riveiro-Falkenbach, Erica
author
Ortiz-Romero, Pablo L
author
Rodríguez-Peralto, José L
author
Maestre L, Lorena
author
Roncador, Giovanna
author
de Agustín Asensio, Juan C
author
Goding, Colin R
author
Eyras, Eduardo
author
Megias Vazquez, Diego
author
Méndez, Raúl
author
Soengas, MS
author
2016
Nuclear 3'-end-polyadenylation is essential for the transport, stability and translation of virtually all eukaryotic mRNAs. Poly(A) tail extension can also occur in the cytoplasm, but the transcripts involved are incompletely understood, particularly in cancer. Here we identify a lineage-specific requirement of the cytoplasmic polyadenylation binding protein 4 (CPEB4) in malignant melanoma. CPEB4 is upregulated early in melanoma progression, as defined by computational and histological analyses. Melanoma cells are distinct from other tumour cell types in their dependency on CPEB4, not only to prevent mitotic aberrations, but to progress through G1/S cell cycle checkpoints. RNA immunoprecipitation, sequencing of bound transcripts and poly(A) length tests link the melanoma-specific functions of CPEB4 to signalling hubs specifically enriched in this disease. Essential in these CPEB4-controlled networks are the melanoma drivers MITF and RAB7A, a feature validated in clinical biopsies. These results provide new mechanistic links between cytoplasmic polyadenylation and lineage specification in melanoma.
Nat Commun. 2016 ;7:13418
2041-1723
http://hdl.handle.net/20.500.12105/7877
27857118
10.1038/ncomms13418
2041-1723
Nature communications
Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers