2024-03-29T05:44:37Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/77972023-10-06T12:14:46Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
00925njm 22002777a 4500
dc
Hurtado, Carolina
author
Bustos, Maria Jose
author
Sabina, Prado
author
Nogal, Maria Luisa
author
Granja, Aitor G
author
Gonzalez Portal, Maria Eugenia
author
Gónzalez-Porqué, Pedro
author
Revilla, Yolanda
author
Carrascosa, Angel L
author
2008
BACKGROUND: Antiviral compounds are needed in the control of many animal and human diseases. METHODS: We analysed the effect of the antitumoural drug lauryl gallate on the infectivity of the African swine fever virus among other DNA (herpes simplex and vaccinia) and RNA (influenza, porcine transmissible gastroenteritis and Sindbis) viruses, paying attention to its effect on the viability of the corresponding host cells. RESULTS: Viral production was strongly inhibited in different cell lines at non-toxic concentrations of the drug (1-10 microM), reducing the titres 3->5 log units depending on the multiplicity of infection. In our model system (African swine fever virus in Vero cells), the addition of the drug 1 h before virus adsorption completely abolished virus productivity in a one-step growth virus cycle. Interestingly, no inhibitory effect was observed when lauryl gallate was added after 5-8 h post-infection. Both cellular and viral DNA synthesis and late viral transcription were inhibited by the drug; however, the early viral protein synthesis and the virus-mediated increase of p53 remained unaffected. Activation of the apoptotic effector caspase-3 was not detected after lauryl gallate treatment of Vero cells. Furthermore, the presence of the drug abrogated the activation of this protease induced by the virus infection. CONCLUSIONS: Lauryl gallate is a powerful antiviral agent against several pathogens of clinical and veterinary importance. The overall results indicate that a cellular factor or function might be the target of the antiviral action of alkyl gallates.
Antivir Ther. 2008;13(7):909-17
1359-6535
http://hdl.handle.net/20.500.12105/7797
19043925
Antiviral therapy
Antiviral activity
protein kinases
apoptosis
Antiviral activity of lauryl gallate against animal viruses