2024-03-28T07:58:32Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/77342023-01-26T12:42:46Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
00925njm 22002777a 4500
dc
Lopez-Galindez, Luis Cecilio
author
Lopez, Juan Antonio
author
Melero, Jose Antonio
author
Fuente, Luis de la
author
Martínez, Concepción
author
Ortin, Juan
author
Perucho, Manuel
author
1988-05
We have applied the RNase A mismatch cleavage method to analyze genetic variability in RNA viruses by using influenza virus as a model system. Uniformly labeled RNA probes synthesized from a cloned hemagglutinin gene of a given viral strain were hybridized to RNA isolated from other strains of characterized or uncharacterized genetic composition. The RNA.RNA heteroduplexes containing a variable number of base mismatches were digested with RNase A, and the resistant products were analyzed by denaturing polyacrylamide gel electrophoresis. We show that many of these single base mismatches are cleaved by RNase A, generating unique and characteristic patterns of resistant RNA fragments specific for each of the different viral strains. Comparative analysis of the cleavage patterns allows a qualitative estimation of the genetic relatedness and evolution of field strains. We also show that cleavage by RNase A at single base mismatches can readily detect and localize point mutations present in monoclonal antibody-resistant variants. This method should have wide applications in the study of RNA viruses, not only for epidemiological analysis but also in some diagnostic problems, such as characterization of phenotypic mutants.
Proc Natl Acad Sci U S A. 1988 May;85(10):3522-6.
0027-8424
http://hdl.handle.net/20.500.12105/7734
3368463
Proceedings of the National Academy of Sciences of the United States of America
Analysis of genetic variability and mapping of point mutations in influenza virus by the RNase A mismatch cleavage method