2024-03-28T10:50:57Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/72462022-10-25T12:05:35Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16978col_20.500.12105_16969col_20.500.12105_2061
00925njm 22002777a 4500
dc
Ramos, Jose Tomas
author
Villar, Sonia
author
Bouza, Emilio
author
Bergon-Sendin, Elena
author
Perez Rivilla, Alfredo
author
Collados, Caridad Tapia
author
Andreu, Mariano
author
Reyes, Candelaria Santana
author
Campos-Herrero, María Isolina
author
López de Heredia, Jon
author
López Herrera, María Cruz
author
Anguita Alonso, Paloma
author
Pallás-Alonso, Carmen Rosa
author
Cuenca-Estrella, Manuel
author
2017-09
An epidemiological, multicenter, noninterventional, observational case-control study was conducted to describe the performance of serum beta-d-glucan (BDG) and Candida PCR in blood, serum, and sterile samples for the diagnosis of invasive candidiasis (IC) in very-low-birth-weight (VLBW) preterm neonates and to compare these techniques with culture of samples from blood and other sterile sites. Seventeen centers participated in the study, and the number of episodes analyzed was 159. A total of 9 episodes of IC from 9 patients (7 confirmed and 2 probable) and 150 episodes of suspected sepsis from 117 controls were identified. The prevalence of IC was 5.7% (95% confidence interval [95% CI], 2.1 to 9.3). The mortality was significantly higher in episodes of IC (44.4%) than in the non-IC episodes (11.1%, P < 0.01). The sensitivity and specificity of the PCR performed on blood/serum samples were 87.5% and 81.6%, respectively. The sensitivity and specificity of the BDG results were lower (75.0% and 64.6%). For cases with negative culture results, the PCR and the BDG results were positive in 27 (17.4%) and 52 (33.5%) episodes, respectively. The presence of multiorgan failure, improvement with empirical antifungal therapy, thrombocytopenia, and Candida colonization were significantly associated (P < 0.01) with PCR or BDG positivity regardless of the results of the cultures. Serum BDG analysis and Candida PCR could be used as complementary diagnostic techniques to detect IC in VLBW neonates.
J Clin Microbiol. 2017 Sep;55(9):2752-2764.
http://hdl.handle.net/20.500.12105/7246
28659321
10.1128/JCM.00496-17
1098-660X
Journal of clinical microbiology
PCR
Invasive candidiasis
Polymerase chain reaction
Serum beta-d-glucan
Performance of a Quantitative PCR-Based Assay and Beta-d-Glucan Detection for Diagnosis of Invasive Candidiasis in Very-Low-Birth-Weight Preterm Neonatal Patients (CANDINEO Study)