2024-03-29T09:10:30Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/68262022-10-05T12:37:28Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16987col_20.500.12105_16961col_20.500.12105_2061
00925njm 22002777a 4500
dc
Almansa, Raquel
author
Antón, Andrés
author
Ramirez, Paula
author
Martin-Loeches, Ignacio
author
Banner, David
author
Pumarola, Tomás
author
Xu, Luoling
author
Blanco, Jesús
author
Ran, Longsi
author
Lopez-Campos, Guillermo
author
Martin-Sanchez, Fernando
author
Socias, Lorenzo
author
Loza, Ana
author
Andaluz, David
author
Maravi, Enrique
author
Gordón, Mónica
author
Gallegos, Maria C
author
Fernandez, Victoria
author
León, Cristobal
author
Merino, Pedro
author
Marcos, Maria Angeles
author
Gandía, Francisco
author
Bobillo, Felipe
author
Resino, Salvador
author
Eiros, José María
author
Castro, Carmen
author
Mateo, Paula
author
Gonzalez-Rivera, Milagros
author
Rello, Jordi
author
de Lejarazu, Raul Ortiz
author
Kelvin, David J
author
Bermejo-Martin, Jesus F
author
2011-08-31
BACKGROUND: Severe disease caused by 2009 pandemic influenza A/H1N1virus is characterized by the presence of hypercytokinemia. The origin of the exacerbated cytokine response is unclear. As observed previously, uncontrolled influenza virus replication could strongly influence cytokine production. The objective of the present study was to evaluate the relationship between host cytokine responses and viral levels in pandemic influenza critically ill patients. METHODS: Twenty three patients admitted to the ICU with primary viral pneumonia were included in this study. A quantitative PCR based method targeting the M1 influenza gene was developed to quantify pharyngeal viral load. In addition, by using a multiplex based assay, we systematically evaluated host cytokine responses to the viral infection at admission to the ICU. Correlation studies between cytokine levels and viral load were done by calculating the Spearman correlation coefficient. RESULTS: Fifteen patients needed of intubation and ventilation, while eight did not need of mechanical ventilation during ICU hospitalization. Viral load in pharyngeal swabs was 300 fold higher in the group of patients with the worst respiratory condition at admission to the ICU. Pharyngeal viral load directly correlated with plasma levels of the pro-inflammatory cytokines IL-6, IL-12p70, IFN-γ, the chemotactic factors MIP-1β, GM-CSF, the angiogenic mediator VEGF and also of the immuno-modulatory cytokine IL-1ra (p < 0.05). Correlation studies demonstrated also the existence of a significant positive association between the levels of these mediators, evidencing that they are simultaneously regulated in response to the virus. CONCLUSIONS: Severe respiratory disease caused by the 2009 pandemic influenza virus is characterized by the existence of a direct association between viral replication and host cytokine response, revealing a potential pathogenic link with the severe disease caused by other influenza subtypes such as H5N1.
BMC Infect Dis. 2011 Aug 31;11:232.
1471-2334
http://hdl.handle.net/20.500.12105/6826
21880131
10.1186/1471-2334-11-232
1471-2334
BMC infectious diseases
Direct association between pharyngeal viral secretion and host cytokine response in severe pandemic influenza