2024-03-28T15:11:07Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/66512023-10-09T09:29:08Zcom_20.500.12105_2152com_20.500.12105_2051com_20.500.12105_2144col_20.500.12105_2153
00925njm 22002777a 4500
dc
Gomez-Garay, Aranzazu
author
Lopez, Juan Antonio
author
Camafeita, Emilio
author
Bueno, Maria Angeles
author
Pintos, Beatriz
author
2013-11-20
UNLABELLED: Quercus suber L. is a forest tree with remarkable ecological, social and economic value in the southern Europe ecosystems. To circumvent the difficulties of breeding such long-lived species like Q. suber in a conventional fashion, clonal propagation of Q. suber elite trees can be carried out, although this process is sometimes unsuccessful. To help decipher the complex program underlying the development of Q. suber somatic embryos from the first early stage until maturity, a proteomic approach based on DIGE and MALDI-MS has been envisaged. Results highlighted several key processes involved in the three developmental stages (proliferative, cotyledonary and mature) of Q. suber somatic embryogenesis studied. Results show that the proliferation stage is characterized by fermentation as an alternative energy source at the first steps of somatic embryo development, as well as by up-regulation of proteins involved in cell division. In this stage reactive oxygen species play a role in proliferation, while other proteins like CAD and PR5 seem to be implied in embryonic competence. In the transition to the cotyledonary stage diverse ROS detoxification enzymes are activated and reserve products (mainly carbohydrates and proteins) are accumulated, whereas energy production is increased probably to participate in the synthesis of primary metabolites such as amino acids and fatty acids. Finally, in the mature stage ethylene accumulation regulates embryo development. BIOLOGICAL SIGNIFICANCE: Quercus suber L. is a forest tree with remarkable ecological, social and economic value in the southern Europe ecosystems. To circumvent the difficulties of breeding such long-lived species like Q. suber in a conventional fashion, clonal propagation of Q. suber elite trees can be carried out, although this process is sometimes unsuccessful. To help decipher the complex program underlying the development of Q. suber somatic embryos from the first early stage until maturity, in deep studies become necessary. This article is part of a Special Issue entitled: Translational Plant Proteomics.
J Proteomics. 2013 Nov 20;93:314-25
18743919
http://hdl.handle.net/20.500.12105/6651
23770300
10.1016/j.jprot.2013.06.006
1876-7737
Journal of proteomics
5-Methyltetrahydropteroyltriglutamate–homocysteine methyltransferase
AA
AFR
AKR
Aldo keto reductase
Ascorbate free radical
Ascorbic acid
CAD
CSE
Cinnamyl alcohol dehydrogenase
Cotyledonar somatic embryos
DHA
DIGE
Dehydroascorbate (synonym: dehydroascorbic acid)
GAPDH
Glyceraldehyde-3-phosphate dehydrogenase
Heat shock protein
Hsp
LEA
Late embryogenesis abundant
MALDI
MSE
MTHP
Mass spectrometry
Mature somatic embryos
PCA
PR protein
PSE
PTM
Pathogenesis-related protein
Post-translational modification
Principal components analysis
Proliferating somatic embryos
Proteomics
Quercus
ROS
Reactive oxygen species
SOD
Somatic embryogenesis
Superoxide dismutase
Proteomic perspective of Quercus suber somatic embryogenesis