2024-03-28T13:39:18Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/66192023-10-05T06:03:35Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
00925njm 22002777a 4500
dc
Mata, Carlos P
author
Luque, Daniel
author
Gómez-Blanco, Josué
author
Rodriguez Martinez, Javier M
author
González, José M
author
Suzuki, Nobuhiro
author
Ghabrial, Said A
author
Carrascosa, José L
author
Trus, Benes L
author
Castón, José R
author
2017-12-08
Unlike their counterparts in bacterial and higher eukaryotic hosts, most fungal viruses are transmitted intracellularly and lack an extracellular phase. Here we determined the cryo-EM structure at 3.7 Å resolution of Rosellinia necatrix quadrivirus 1 (RnQV1), a fungal double-stranded (ds)RNA virus. RnQV1, the type species of the family Quadriviridae, has a multipartite genome consisting of four monocistronic segments. Whereas most dsRNA virus capsids are based on dimers of a single protein, the ~450-Å-diameter, T = 1 RnQV1 capsid is built of P2 and P4 protein heterodimers, each with more than 1000 residues. Despite a lack of sequence similarity between the two proteins, they have a similar α-helical domain, the structural signature shared with the lineage of the dsRNA bluetongue virus-like viruses. Domain insertions in P2 and P4 preferential sites provide additional functions at the capsid outer surface, probably related to enzyme activity. The P2 insertion has a fold similar to that of gelsolin and profilin, two actin-binding proteins with a function in cytoskeleton metabolism, whereas the P4 insertion suggests protease activity involved in cleavage of the P2 383-residue C-terminal region, absent in the mature viral particle. Our results indicate that the intimate virus-fungus partnership has altered the capsid genome-protective and/or receptor-binding functions. Fungal virus evolution has tended to allocate enzyme activities to the virus capsid outer surface.
PLoS Pathog. 2017 Dec 8;13(12):e1006755.
1553-7374
http://hdl.handle.net/20.500.12105/6619
29220409
10.1371/journal.ppat.1006755
1553-7374
PLoS pathogens
Acquisition of functions on the outer capsid surface during evolution of double-stranded RNA fungal viruses