2024-03-29T11:25:02Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/56532021-05-18T14:46:00Zcom_20.500.12105_2174com_20.500.12105_2051com_20.500.12105_2173col_20.500.12105_2175
00925njm 22002777a 4500
dc
Zhou, Jing
author
Benito-Martin, Alberto
author
Mighty, Jason
author
Chang, Lynne
author
Ghoroghi, Shima
author
Wu, Hao
author
Wong, Madeline
author
Guariglia, Sara
author
Baranov, Petr
author
Young, Michael
author
Gharbaran, Rajendra
author
Emerson, Mark
author
Mark, Milica Tesic
author
Molina, Henrik
author
Canto-Solar, M Valeria
author
Peinado Selgas , Hector
author
Redenti, Stephen
author
2018-02-12
A range of cell types, including embryonic stem cells, neurons and astrocytes have been shown to release extracellular vesicles (EVs) containing molecular cargo. Across cell types, EVs facilitate transfer of mRNA, microRNA and proteins between cells. Here we describe the release kinetics and content of EVs from mouse retinal progenitor cells (mRPCs). Interestingly, mRPC derived EVs contain mRNA, miRNA and proteins associated with multipotency and retinal development. Transcripts enclosed in mRPC EVs, include the transcription factors Pax6, Hes1, and Sox2, a mitotic chromosome stabilizer Ki67, and the neural intermediate filaments Nestin and GFAP. Proteomic analysis of EV content revealed retinogenic growth factors and morphogen proteins. mRPC EVs were shown to transfer GFP mRNA between cell populations. Finally, analysis of EV mediated functional cargo delivery, using the Cre-loxP recombination system, revealed transfer and uptake of Cre+ EVs, which were then internalized by target mRPCs activating responder loxP GFP expression. In summary, the data supports a paradigm of EV genetic material encapsulation and transfer within RPC populations. RPC EV transfer may influence recipient RPC transcriptional and post-transcriptional regulation, representing a novel mechanism of differentiation and fate determination during retinal development.
Sci Rep. 2018;8(1):2823.
2045-2322
http://hdl.handle.net/20.500.12105/5653
29434302
10.1038/s41598-018-20421-1
2045-2322
Scientific reports
ROD PHOTORECEPTOR DIFFERENTIATION
STEM-CELLS
IN-VITRO
GENE-EXPRESSION
MESSENGER-RNA
FATE DETERMINATION
MEDIATED TRANSFER
APOPTOTIC BODIES
MOUSE RETINA
BODY-FLUIDS
Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins