2024-03-29T14:21:32Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/52592023-10-03T13:45:03Zcom_20.500.12105_2145com_20.500.12105_2051com_20.500.12105_2144com_20.500.12105_2152col_20.500.12105_2146col_20.500.12105_2153
00925njm 22002777a 4500
dc
Ezkurdia, Iakes
author
Calvo, Enrique
author
Del Pozo, Angela
author
Vazquez, Jesus
author
Valencia, Alfonso
author
Tress, Michael L.
author
2015
The authors have carried out an investigation of the two draft maps of the human proteome published in 2014 in Nature. The findings include an abundance of poor spectra, low-scoring peptide-spectrum matches and incorrectly identified proteins in both these studies, highlighting clear issues with the application of false discovery rates. This noise means that the claims made by the two papers - the identification of high numbers of protein coding genes, the detection of novel coding regions and the draft tissue maps themselves - should be treated with considerable caution. The authors recommend that clinicians and researchers do not use the unfiltered data from these studies. Despite this these studies will inspire further investigation into tissue-based proteomics. As long as this future work has proper quality controls, it could help produce a consensus map of the human proteome and improve our understanding of the processes that underlie health and disease.
Expert Rev Proteomics. 2015; 12(6):579-93
1478-9450
http://hdl.handle.net/20.500.12105/5259
26496066
10.1586/14789450.2015.1103186
1744-8387
Expert Review of Proteomics
Clinical applications
false discovery rates
human proteome
protein coding genes
proteomics
FALSE DISCOVERY RATE
SORF-ENCODED POLYPEPTIDES
MASS-SPECTROMETRY
MISSING PROTEINS
CODING GENES
HUMAN GENOME
IDENTIFICATION
PROJECT
DATABASE
PEPTIDE
The potential clinical impact of the release of two drafts of the human proteome