2024-03-29T02:04:05Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/144162023-10-10T10:24:35Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16985col_20.500.12105_16982col_20.500.12105_16958col_20.500.12105_2061
00925njm 22002777a 4500
dc
Pacios, Olga
author
Fernández-García, Laura
author
Bleriot, Inés
author
Blasco, Lucia
author
Ambroa, Antón
author
López, María
author
Ortiz-Cartagena, Concha
author
Fernández-Cuenca, Felipe
author
Oteo-Iglesias, Jesus
author
Pascual, Álvaro
author
Martínez-Martínez, Luis
author
Domingo-Calap, Pilar
author
Tomás, María
author
2022
Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immunocompromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically characterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM-VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM-VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylogenetic study performed with other Tevenvirinae phages showed a close common ancestor. However, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nucleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.
Viruses. 2022;14(1):6.
http://hdl.handle.net/20.500.12105/14416
35062209
10.3390/v14010006
1999-4915
Viruses
Klebsiella pneumoniae
L-shaped tail fiber
Genomic annotation
Homing endonucleases
Lytic phages
Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13