2024-03-29T13:43:40Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/141602023-04-28T18:08:19Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16961col_20.500.12105_2061
00925njm 22002777a 4500
dc
Fehér, Csaba
author
Pastor-Ibáñez, Roque
author
Leal, Lorna
author
Plana, Montserrat
author
Arnedo, Mireia
author
van den Ham, Henk-Jan
author
Andeweg, Arno C
author
Gruters, Rob A
author
Díez-Fuertes, Francisco
author
Alcamí, José
author
Aloy, Patrick
author
García, Felipe
author
2021-07-19
Systems vaccinology has seldomly been used in therapeutic HIV-1 vaccine research. Our aim was to identify early gene 'signatures' that predicted virus load control after analytical therapy interruption (ATI) in participants of a dendritic cell-based HIV-1 vaccine trial (DCV2). mRNA and miRNA were extracted from frozen post-vaccination PBMC samples; gene expression was determined by microarray method. In gene set enrichment analysis, responders showed an up-regulation of 14 gene sets (TNF-alpha/NFkB pathway, inflammatory response, the complement system, Il6 and Il2 JAK-STAT signaling, among others) and a down-regulation of 7 gene sets (such as E2F targets or interferon alpha response). The expression of genes regulated by three (miR-223-3p, miR-1183 and miR-8063) of the 9 differentially expressed miRNAs was significantly down-regulated in responders. The deregulation of certain gene sets related to inflammatory processes seems fundamental for viral control, and certain miRNAs may be important in fine-tuning these processes.
Vaccines (Basel). 2021;9(7):799.
2076-393X
http://hdl.handle.net/20.500.12105/14160
34358215
10.3390/vaccines9070799
Vaccines
Dendritic cell-based therapeutic HIV-1 vaccine
Differential gene expression
Gene set enrichment analysis
miRNA
mMRA
Association of Transcriptomic Signatures of Inflammatory Response with Viral Control after Dendritic Cell-Based Therapeutic Vaccination in HIV-1 Infected Individuals