2024-03-28T15:42:49Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/141252023-10-06T11:10:31Zcom_20.500.12105_2102com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2103
00925njm 22002777a 4500
dc
Melero-Fernandez de Mera, Raquel M.
author
VillaseƱor, Alma
author
Rojo, David
author
CarriĆ³n-Navarro, Josefa
author
Gradillas, Ana
author
Ayuso-Sacido, Angel
author
Barbas, Coral
author
2022-01-21
Glioblastoma (GBM) is one of the most malignant central nervous system tumor types. Comparative analysis of GBM tissues has rendered four major molecular subtypes. From them, two molecular subtypes are mainly found in their glioblastoma cancer stem-like cells (GSCs) derived in vitro: proneural (PN) and mesenchymal (MES) with nodular (MES-N) and semi-nodular (MES-SN) disseminations, which exhibit different metabolic, growth, and malignancy properties. Many studies suggest that cancer cells communicate between them, and the surrounding microenvironment, via exosomes. Identifying molecular markers that allow the specific isolation of GSC-derived exosomes is key in the development of new therapies. However, the differential exosome composition produced by main GSCs remains unknown. The aim of this study was to determine ceramide (Cer) composition, one of the critical lipids in both cells and their cell-derived exosomes, from the main three GSC phenotypes using mass spectrometry-based lipidomics. GSCs from human tissue samples and their cell-derived exosomes were measured using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) in an untargeted analysis. Complete characterization of the ceramide profile, in both cells and cell-derived exosomes from GSC phenotypes, showed differential distributions among them. Results indicate that such differences of ceramide are chain-length dependent. Significant changes for the C16 Cer and C24:1 Cer and their ratio were observed among GSC phenotypes, being different for cells and their cell-derived exosomes.
Front Oncol. 2022;11:788100.
2234-943X
http://hdl.handle.net/20.500.12105/14125
35127492
10.3389/fonc.2021.788100
Frontiers in Oncology
LC-MS
Cancer stem cells
Ceramides
Exosomes
Glioblastoma
Mesenchymal Phenotype
Proneural phenotype
Untargeted lipidomics
Ceramide Composition in Exosomes for Characterization of Glioblastoma Stem-Like Cell Phenotypes.