2024-03-29T08:47:12Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/129552022-11-07T10:50:00Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16990col_20.500.12105_2061
00925njm 22002777a 4500
dc
García-Suárez, María Del Mar
author
González-Rodríguez, Irene
author
Cima-Cabal, María Dolores
author
Yuste, Jose Enrique
author
Vazquez, Fernando
author
Santiago, Enrique
author
2019-11-18
Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzymes for Restriction Fragment Length Polymorphism-RFLP typing, in order to maximize the discrimination between different serotypes. DNA sequences of 90 serotypes for the region between dexB and aliA genes were compiled, and a computer screening of restriction enzymes was performed. The wzg-wzh-wzd-wze region and Sse9I restriction predicted unique PCR-RFLP patterns for 39 serotypes and eight serogroups. A second restriction enzyme resolved fragment specific patterns for 25 serotypes. The method was tested with 98 serotype-unknown clinical isolates. PCR-RFLP analysis deduced correct serotypes that were confirmed by Quellung reaction for 78.5% of the isolates.
Diagnostics (Basel). 2019; 9(4):196
2075-4418
http://hdl.handle.net/20.500.12105/12955
31752204
10.3390/diagnostics9040196
Diagnostics (Basel)
PCR-RFLP
Streptococcus pneumoniae
Serotype
Identification of Pneumococcal Serotypes by PCR-Restriction Fragment Length Polymorphism.