2024-03-29T05:17:24Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/114972023-10-06T14:37:50Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
00925njm 22002777a 4500
dc
Olías-Molero, A I
author
Moreno-Iruela, Inmaculada
author
Corral, María J
author
Jiménez-Antón, M D
author
Day, M J
author
Domínguez, M
author
Alunda, José M
author
2020
Leishmania infantum is the etiological agent of zoonotic visceral leishmaniasis. In endemic areas, canine infections are considered the main source of infection for human populations. Therefore, any control of human leishmaniasis must include the control of canine infections. Chemotherapy of leishmaniasis is inadequate and canine immunoprophylaxis has important limitations. Reports on the response of infected dogs are abundant but no clear picture of immune events has emerged. To shed some light on these shortcomings the specific IgG subclass response was followed in 20 Beagle dogs experimentally infected with L. infantum using monoclonal antibodies (MAb) specific for canine IgG1, IgG2, IgG3 and IgG4, along with ELISA and flow cytometry. Results showed that parasitic infection elicits a general response of all IgG subclasses, with a predominant IgG1 response and without any evidence of IgG1/IgG2 dichotomy. These findings suggest that the inconsistent results reported previously could be related to the lack of specific reagents and not to the actual differences in the immune response of infected animals. Differential IgG subclass reactivity in ELISA and cytometry and the analysis of the reacting antigens could facilitate the diagnosis and prognosis of the disease and provide a useful tool for adequate therapeutics and vaccine development against leishmaniasis.
Sci Rep. 2020 Nov 2;10(1):18826
http://hdl.handle.net/20.500.12105/11497
33139752
10.1038/s41598-020-75569-6
2045-2322
Scientific reports
Infection of dogs by Leishmania infantum elicits a general response of IgG subclasses.