2024-03-29T07:32:34Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/104452022-10-25T08:59:11Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
00925njm 22002777a 4500
dc
Sanz, Juan Carlos
author
De Ory, Fernando de
author
Guisasola, Maria Eulalia
author
Balfagon, Pilar
author
2018-01
Serology for type-specific herpes simplex virus (HSV) is based on the use of the respective glycoprotein G (gG).
Chemiluminescent immunoassay (CLIA; BIO-FLASH® , Biokit, Spain), ELISA (HerpeSelect® , Focus, USA), and immunoblot (IB; Virotech, Germany) for detecting HSV-1- and HSV-2-specific IgG were compared using 384 serum samples received for HSV serology. The samples were classified as positive or negative according to a consensus criterion.
For HSV-1, 262 samples were positive and 118 were negative (four samples were unclassifiable). IB showed agreement, sensitivity, and specificity values of 98.68%, 98.47% and 99.15%, respectively. The corresponding figures for CLIA and ELISA were 98.95%, 99.24% and 98.31%, and 98.16%, 99.62% and 94.92%, respectively. For HSV-2, 106 samples were positive and 278 were negative. Agreement, sensitivity, and specificity of IB were 99.48%, 98.11%, and 100%, respectively. The corresponding figures for CLIA and ELISA were 99.48%, 99.06% and 99.64%, and 98.18%, 99.06% and 97.84%, respectively.
The three methods showed excellent and equivalent performance characteristics for the detection of type-specific IgG to HSV-1 and HSV-2.
J Clin Lab Anal . 2018 Jan;32(1):e22203.
0887-8013
http://hdl.handle.net/20.500.12105/10445
28332725
10.1002/jcla.22203
1098-2825
Journal of clinical laboratory analysis
Comparison of commercial methods of immunoblot, ELISA, and chemiluminescent immunoassay for detecting type-specific herpes simplex viruses-1 and -2 IgG.