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dc.contributor.authorMarinaro, Federica
dc.contributor.authorMacías-García, Beatriz
dc.contributor.authorSánchez-Margallo, Francisco Miguel
dc.contributor.authorBlázquez, Rebeca
dc.contributor.authorÁlvarez, Verónica
dc.contributor.authorMatilla, Elvira
dc.contributor.authorHernández, Nuria
dc.contributor.authorGomez-Serrano, Maria 
dc.contributor.authorJorge, Inmaculada 
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorGonzález-Fernández, Lauro
dc.contributor.authorPericuesta, Eva
dc.contributor.authorGutiérrez-Adán, Alfonso
dc.contributor.authorCasado, Javier G
dc.identifier.citationBiol Reprod. 2019; 100(5):1180-92es_ES
dc.description.abstractAdvanced age is a risk factor undermining women's fertility. Hence, the optimization of assisted reproduction techniques is an interdisciplinary challenge that requires the improvement of in vitro culture systems. Here, we hypothesize that supplementation of embryo culture medium with extracellular vesicles from endometrial-derived mesenchymal stem cells (EV-endMSCs) may have a positive impact on the embryo competence of aged oocytes. In this work, 24 weeks old B6D2 female mice were used as egg donors and in vitro fertilization assays were performed using males from the same strain (8-12 weeks); the presumptive zygotes were incubated in the presence of 0, 10, 20, 40, or 80 μg/ml of EV-endMSCs. The results from the proteomic analysis of EV-endMSCs and the classification by Reactome pathways allowed us to identify proteins closely related with the fertilization process. Moreover, in our aged murine model, the supplementation of the embryo culture medium with EV-endMSCs improved the developmental competence of the embryos as well as the total blastomere count. Finally, gene expression analysis of murine blastocysts showed significant changes on core genes related to cellular response to oxidative stress, metabolism, placentation, and trophectoderm/inner cell mass formation. In summary, we demonstrate that EV-endMSCs increase the quality of the embryos, and according to proteomic and genomic analysis, presumably by modulating the expression of antioxidant enzymes and promoting pluripotent activity. Therefore, EV-endMSCs could be a valuable tool in human assisted reproduction improving the developmental competence of aged oocytes and increasing the odds of implantation and subsequent delivery.es_ES
dc.description.sponsorshipThis work was supported by one grant "Miguel Servet I" from Instituto de Salud Carlos III to JGC (CD17/00021 co-financed by FSE); one grant MAFRESA S.L. to FM.es_ES
dc.publisherOxford University Presses_ES
dc.subjectembryo culturees_ES
dc.subjectgene expressiones_ES
dc.subjectoxidative stresses_ES
dc.subjectstem cellses_ES
dc.titleExtracellular vesicles derived from endometrial human mesenchymal stem cells enhance embryo yield and quality in an aged murine model†es_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.identifier.journalBiology of reproductiones_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional