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dc.contributor.authorHagensen, Mette K
dc.contributor.authorMortensen, Martin B
dc.contributor.authorKjolby, Mads
dc.contributor.authorPalmfeldt, Johan
dc.contributor.authorBentzon, Jacob F 
dc.contributor.authorGregersen, Soeren
dc.date.accessioned2020-05-07T10:49:13Z
dc.date.available2020-05-07T10:49:13Z
dc.date.issued2019-07
dc.identifier.citationAtherosclerosis. 2019; 286:156-162es_ES
dc.identifier.issn0021-9150es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9946
dc.description.abstractBACKGROUND AND AIMS: Type 1 diabetes accelerates the development of atherosclerotic cardiovascular diseases. Retention of low-density lipoprotein (LDL) in the arterial wall is a causal step in atherogenesis, but it is unknown whether diabetes alters the propensity of LDL for retention. The present study investigated whether LDL from type 1 diabetic and healthy non-diabetic subjects differed in their ability to bind to the arterial wall in a type 1 diabetic mouse model. METHODS: Fluorescently-labeled LDL obtained from type 1 diabetic patients or healthy controls was injected into mice with type 1 diabetes. The amount of retained LDL in the atherosclerosis-prone inner curvature of the aortic arch was quantified by fluorescence microscopy. Healthy control LDL was in vitro glycated, analyzed for protein glycation by LC-MS/MS, and tested for retention propensity. RESULTS: Retention of LDL from type 1 diabetic patients was 4.35-fold higher compared to LDL from nondiabetic subjects. Nuclear magnetic resonance (NMR) spectroscopy analysis of LDL revealed no differences in the concentration of the atherogenic small dense LDL between type 1 diabetic and non-diabetic subjects. In vitro glycation of LDL from a non-diabetic subject increased retention compared to non-glycated LDL. LC-MS/MS revealed four new glycated spots in the protein sequence of ApoB of in vitro glycated LDL. CONCLUSIONS: LDL from type 1 diabetic patients showed increased retention at atherosclerosis-prone sites in the arterial wall of diabetic mice. Glycation of LDL is one modification that may increase retention, but other, yet unknown, mechanisms are also likely to contribute.es_ES
dc.description.sponsorshipThis work was supported by The Arvid Nilssons Foundation, Fonden til Laegevidenskabens Fremme, Snedkermester Sophus Jacobsen & Hustru Astrid Jacobsens Fond, Laegeforeningen, The Novo Nordisk Foundation, John and Birthe Meyer Foundation, The Danish Council for Independent Research (10-093408) and Danish Diabetes Academy. AGRADECIENTOS:es_ES
dc.language.isoenges_ES
dc.publisherElsevier es_ES
dc.type.hasVersionAMes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAtherosclerosises_ES
dc.subjectLow density lipoproteines_ES
dc.subjectRetentiones_ES
dc.subjectType 1 diabeteses_ES
dc.titleIncreased retention of LDL from type 1 diabetic patients in atherosclerosis-prone areas of the murine arterial walles_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID30871723es_ES
dc.format.volume286es_ES
dc.format.page156-162es_ES
dc.identifier.doi10.1016/j.atherosclerosis.2019.02.027es_ES
dc.embargo.terms2020-07-01es_ES
dc.identifier.e-issn1879-1484es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.atherosclerosis.2019.02.027es_ES
dc.identifier.journalAtherosclerosises_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Patología Experimental de la Aterosclerosises_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsopen accesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 Internacional