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dc.contributor.authorHagensen, Mette K
dc.contributor.authorMortensen, Martin Bødtker
dc.contributor.authorKjolby, Mads
dc.contributor.authorStillits, Ninna L
dc.contributor.authorSteffensen, Lasse B
dc.contributor.authorBentzon, Jacob F
dc.identifier.citationAtherosclerosis. 2017; 263:7-14es_ES
dc.description.abstractBACKGROUND AND AIMS: Individuals with type 1 diabetes mellitus are at high risk of developing atherosclerotic cardiovascular disease, but the underlying mechanisms by which type 1 diabetes accelerates atherosclerosis remain unknown. Increased retention of low-density lipoprotein (LDL) in atherosclerosis-prone sites of the diabetic vascular wall has been suggested, but direct evidence is lacking. In the present study, we investigated whether retention of LDL is increased in atherosclerotic-prone areas using a murine model of type 1 diabetes. METHODS: Fluorescently-labeled human LDL from healthy non-diabetic individuals was injected into diabetic Ins2Akita mice and non-diabetic, wild-type littermates. The amount of retained LDL after 24 h was quantified by fluorescence microscopy of cryosections and by scans of en face preparations. Vascular gene expression in the inner curvature of the aortic arch was analyzed by microarray and quantitative polymerase chain reaction. RESULTS: LDL retention was readily detected in atherosclerosis-prone areas of the aortic arch being located in both intimal and medial layers. Quantitative microscopy revealed 8.1-fold more retained LDL in type 1 diabetic mice compared to wild-type mice. These findings were confirmed in independent experiments using near-infrared scanning of en face preparations of the aorta. Diabetic status did not affect arterial expression of genes known to be involved in LDL retention. CONCLUSIONS: Type 1 diabetes increases the ability of the vascular wall to retain LDL in mice. These changes could contribute to the increased atherosclerotic burden seen in type 1 diabetic patients.es_ES
dc.description.sponsorshipThis work was supported by The Arvid Nilssons Foundation, Fonden til Laegevidenskabens Fremme, Snedkermester Sophus Jacobsen & Hustru Astrid Jacobsens Fond, Laegeforeningen, The Novo Nordisk Foundation and The Danish Council for Independent Research (10-093408).es_ES
dc.subjectLow-density lipoproteines_ES
dc.subjectType 1 diabeteses_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAorta es_ES
dc.subject.meshAorta, Thoracic es_ES
dc.subject.meshArteries es_ES
dc.subject.meshAtherosclerosis es_ES
dc.subject.meshBlood Glucose es_ES
dc.subject.meshCarotid Intima-Media Thickness es_ES
dc.subject.meshDiabetes Mellitus, Experimental es_ES
dc.subject.meshDiabetes Mellitus, Type 1 es_ES
dc.subject.meshDisease Models, Animales_ES
dc.subject.meshFemale es_ES
dc.subject.meshFluorescent Dyes es_ES
dc.subject.meshGene Expression Profiling es_ES
dc.subject.meshHumans es_ES
dc.subject.meshHypercholesterolemia es_ES
dc.subject.meshHyperglycemia es_ES
dc.subject.meshLipoproteins, LDL es_ES
dc.subject.meshMale es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred C57BL es_ES
dc.subject.meshMicroscopy, Fluorescence es_ES
dc.subject.meshMutation es_ES
dc.subject.meshTissue Array Analysis es_ES
dc.titleType 1 diabetes increases retention of low-density lipoprotein in the atherosclerosis-prone area of the murine aortaes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.repisalud.orgCNICCNIC::Grupos de investigación::Patología Experimental de la Aterosclerosises_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional