Mostrar el registro sencillo del ítem
dc.contributor.author | Ochando, Jordi | |
dc.contributor.author | Conde-San Román, Patricia | |
dc.date.accessioned | 2020-04-30T07:01:33Z | |
dc.date.available | 2020-04-30T07:01:33Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | J Vis Exp. 2017 Jun 7;(124):54242. | es_ES |
dc.identifier.issn | 1940-087X | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/9814 | |
dc.description.abstract | Macrophage accumulation in transplanted organs has long been recognized as a feature of allograft rejection1. Immunogenic monocytes infiltrate the allograft early after transplantation, mount a graft reactive response against the transplanted organ, and initiate organ rejection2. Recent data suggest that suppressive macrophages facilitate successful long-term transplantation3 and are required for the induction of transplantation tolerance4. This suggests a multidimensional concept of macrophage ontogeny, activation, and function, which demands a new roadmap for the isolation and analysis of macrophage function5. Due to the plasticity of macrophages, it is necessary to provide a methodology to isolate and characterize macrophages, depending on the tissue environment, and to define their functions according to different scenarios. Here, we describe a protocol for immune characterization of graft-infiltrating macrophages and the methods we used to functionally evaluate their capacity to inhibit CD8+ T proliferation and to promote CD4+Foxp3+ Treg expansion in vitro. | es_ES |
dc.description.sponsorship | We acknowledge the technical contributions of the Flow Cytometry, Microsurgery, and Bio- repository/Pathology Centers of Research Excellence at Mount Sinai. This work was supported by the COST Action BM1305: Action to Focus and Accelerate Cell Tolerogenic Therapies (A FACTT), the Mount Sinai Recanati/Miller Transplantation Institute developmental funds, Ministerio de Ciencia e Innovacion SAF2013-48834-R and SAF2016-80031-R J.O. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | JoVE | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Graft Rejection | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Macrophages | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | T-Lymphocytes, Regulatory | es_ES |
dc.title | Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity | es_ES |
dc.type | journal article | es_ES |
dc.identifier.pubmedID | 28654060 | es_ES |
dc.format.number | 124 | es_ES |
dc.identifier.doi | 10.3791/54242 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1940-087X | es_ES |
dc.relation.publisherversion | https://doi.org/10.3791/54242 | es_ES |
dc.identifier.journal | Journal of visualized experiments: JoVE | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/BM1305 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/SAF2013-48834-R | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/SAF2016-80031-R | es_ES |
dc.rights.accessRights | open access | es_ES |