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dc.contributor.authorCano-Ochando, Jordi 
dc.contributor.authorConde-San Román, Patricia 
dc.date.accessioned2020-04-30T07:01:33Z
dc.date.available2020-04-30T07:01:33Z
dc.date.issued2017
dc.identifier.citationJ Vis Exp. 2017 Jun 7;(124):54242.es_ES
dc.identifier.issn1940-087Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9814
dc.description.abstractMacrophage accumulation in transplanted organs has long been recognized as a feature of allograft rejection1. Immunogenic monocytes infiltrate the allograft early after transplantation, mount a graft reactive response against the transplanted organ, and initiate organ rejection2. Recent data suggest that suppressive macrophages facilitate successful long-term transplantation3 and are required for the induction of transplantation tolerance4. This suggests a multidimensional concept of macrophage ontogeny, activation, and function, which demands a new roadmap for the isolation and analysis of macrophage function5. Due to the plasticity of macrophages, it is necessary to provide a methodology to isolate and characterize macrophages, depending on the tissue environment, and to define their functions according to different scenarios. Here, we describe a protocol for immune characterization of graft-infiltrating macrophages and the methods we used to functionally evaluate their capacity to inhibit CD8+ T proliferation and to promote CD4+Foxp3+ Treg expansion in vitro.es_ES
dc.description.sponsorshipWe acknowledge the technical contributions of the Flow Cytometry, Microsurgery, and Bio- repository/Pathology Centers of Research Excellence at Mount Sinai. This work was supported by the COST Action BM1305: Action to Focus and Accelerate Cell Tolerogenic Therapies (A FACTT), the Mount Sinai Recanati/Miller Transplantation Institute developmental funds, Ministerio de Ciencia e Innovacion SAF2013-48834-R and SAF2016-80031-R J.O.es_ES
dc.language.isoenges_ES
dc.publisherJoVE es_ES
dc.type.hasVersionVoRes_ES
dc.subject.meshAnimals es_ES
dc.subject.meshGraft Rejection es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMacrophages es_ES
dc.subject.meshMice es_ES
dc.subject.meshT-Lymphocytes, Regulatory es_ES
dc.titleFunctional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunityes_ES
dc.typejournal articlees_ES
dc.identifier.pubmedID28654060es_ES
dc.format.number124es_ES
dc.identifier.doi10.3791/54242es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1940-087Xes_ES
dc.relation.publisherversionhttps://doi.org/10.3791/54242es_ES
dc.identifier.journalJournal of visualized experiments: JoVEes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/BM1305es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/SAF2013-48834-Res_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/SAF2016-80031-Res_ES
dc.rights.accessRightsopen accesses_ES


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