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dc.contributor.authorTa Tang, Thuy-Huong 
dc.contributor.authorLópez-Vélez, Rogelio
dc.contributor.authorLanza-Suarez, Marta 
dc.contributor.authorShelley, Anthony John
dc.contributor.authorRubio Muñoz, Jose Miguel 
dc.contributor.authorLuz, Sérgio Luiz Bessa
dc.date.accessioned2020-04-30T06:58:16Z
dc.date.available2020-04-30T06:58:16Z
dc.date.issued2010-09
dc.identifier.citationInstituto Oswaldo Cruz, Ministério da Saúdees_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9811
dc.description.abstractWe present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly sensitive and specific and it detects low-level infections in both humans and vectors. No cross-amplifications were observed with various other blood parasites and no false-positive results were obtained with the nested PCR. The method works efficiently with whole-blood, blood-spot and skin biopsy samples. Our method may thus be suitable for assessing the efficacy of filaria control programmes in Amazonia by recording parasite infections in both the human host and the vector. By specifically differentiating the major sympatric species of filaria, this technique could also enhance epidemiological research in the region.es_ES
dc.language.isoenges_ES
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúdees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnimals es_ES
dc.subject.meshBrazil es_ES
dc.subject.meshDNA, Protozoan es_ES
dc.subject.meshDNA, Ribosomal es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMansonella es_ES
dc.subject.meshOnchocerca volvulus es_ES
dc.subject.meshRNA, Ribosomal, 18S es_ES
dc.subject.meshReproducibility of Results es_ES
dc.subject.meshSpecies Specificity es_ES
dc.subject.meshPolymerase Chain Reaction es_ES
dc.titleNested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Regiones_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID20945000es_ES
dc.format.volume105es_ES
dc.format.number6es_ES
dc.format.page823-8es_ES
dc.identifier.doi10.1590/s0074-02762010000600016es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1678-8060es_ES
dc.relation.publisherversionhttps://doi.org/10.1590/s0074-02762010000600016es_ES
dc.identifier.journalMemorias do Instituto Oswaldo Cruzes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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