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dc.contributor.authorLópez, Daniel 
dc.contributor.authorCalero, Olga 
dc.contributor.authorJimenez, Mercedes 
dc.contributor.authorGarcía-Calvo, Margarita
dc.contributor.authorDel Val, Margarita
dc.identifier.citationJ Biol Chem. 2006 Oct 13;281(41):30315-8. Epub 2006 Jul 21.es_ES
dc.description.abstractMass spectrometry (MS)-based methods coupled to reverse phase chromatography separation are a useful technology to analyze complex peptide pools that are comprised of different peptides with unrelated sequences. In antigen presentation, proteasomes generate a set of short peptides that are closely related and overlapping and in some instances may even have identical retention times and identical masses. In these situations, micro-liquid chromatography-MS/MS focused on each theoretical parent ion followed by manual interpretation optimizes the identification of generated peptides. The results suggest that the degradation of short antigens by the proteasome occurs by sequential cleavage.es_ES
dc.description.sponsorshipThis work was supported by grants from the Programa Ramón y Cajal, Comunidad de Madrid, Instituto de Salud Carlos III, Fundación FIPSE, and Fondo de Investigaciones Sanitarias de la SS (to D. L.) and from the Ministerio de Educación y Ciencia, Comunidad de Madrid, and Instituto de Salud Carlos III (to M. D. V.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.es_ES
dc.publisherAmerican Society for Biochemistry and Molecular Biologyes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshAmino Acid Sequence es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAntigen Presentation es_ES
dc.subject.meshAntigens, Viral es_ES
dc.subject.meshChromatography es_ES
dc.subject.meshChromatography, Liquid es_ES
dc.subject.meshMass Spectrometry es_ES
dc.subject.meshMolecular Sequence Data es_ES
dc.subject.meshPeptides es_ES
dc.subject.meshProteasome Endopeptidase Complex es_ES
dc.subject.meshRabbits es_ES
dc.titleAntigen processing of a short viral antigen by proteasomeses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderMinisterio de Educación y Ciencia (España)es_ES
dc.identifier.journalThe Journal of biological chemistryes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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