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dc.contributor.authorBonzon-Kulichenko, Elena 
dc.contributor.authorCamafeita, Emilio 
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorGómez-Serrano, María 
dc.contributor.authorJorge, Inmaculada 
dc.contributor.authorCalvo, Enrique 
dc.contributor.authorNunez, Estefania 
dc.contributor.authorTrevisan-Herraz, Marco 
dc.contributor.authorBagwan, Navratan 
dc.contributor.authorBárcena, José Antonio
dc.contributor.authorPeral, Belén
dc.contributor.authorVazquez, Jesus 
dc.date.accessioned2020-04-21T10:38:19Z
dc.date.available2020-04-21T10:38:19Z
dc.date.issued2020-03
dc.identifier.citationJ Proteomics. 2020; 214:103624es_ES
dc.identifier.issn1874-3919es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9651
dc.description.abstractChanges in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women.es_ES
dc.description.sponsorshipThis study was supported by competitive grants from the Spanish Ministry of Science, Innovation and Universities (BIO2015-67580-P, PGC2018-097019-B-I00 and RTI2018-093864-B-100),the Ministry of Economy, Industry and Competitiveness (BFU2016-80006-P) through the Carlos III Institute of Health-Fondo de InvestigaciónSanitaria grant PRB3 (IPT17/0019 -ISCIII-SGEFI / ERDF, ProteoRed), the Fundació MaratóTV3 (grant 122/C/2015) and “la Caixa” Banking Foundation (project code HR17-00247). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia, Innovación y Universidades (MCNU) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505)es_ES
dc.language.isoenges_ES
dc.publisherElsevier es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAdipose tissuees_ES
dc.subjectCys oxidationes_ES
dc.subjectFASILOXes_ES
dc.subjectFilter-aided sample preparationes_ES
dc.subjectSexual dimorphismes_ES
dc.subjectThiol redox proteomees_ES
dc.titleImproved integrative analysis of the thiol redox proteome using filter-aided sample preparationes_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID31874222es_ES
dc.format.volume214es_ES
dc.format.page103624es_ES
dc.identifier.doi10.1016/j.jprot.2019.103624es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) 
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderFundación La Caixa 
dc.contributor.funderFundación La Marató TV3 
dc.contributor.funderFundación ProCNIC 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1876-7737es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.jprot.2019.103624es_ES
dc.identifier.journalJournal of proteomicses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2015-67580-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PGC2018-097019-B-I00es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RTI2018-093864-B-100es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BFU2016-80006-Pes_ES
dc.rights.accessRightsopen accesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional