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dc.contributor.author | Ferrandiz-Avellano, Maria-Jose | |
dc.contributor.author | Martin-Galiano, Antonio Javier | |
dc.contributor.author | Arnanz, Cristina | |
dc.contributor.author | Camacho-Soguero, Isabel | |
dc.contributor.author | Tirado-Velez, JM | |
dc.contributor.author | de la Campa, Adela G | |
dc.date.accessioned | 2020-04-08T12:24:55Z | |
dc.date.available | 2020-04-08T12:24:55Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Nucleic Acids Res. 2016 Sep 6;44(15):7292-303. | es_ES |
dc.identifier.issn | 0305-1048 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/9487 | |
dc.description.abstract | We studied the transcriptional response to an increase in DNA supercoiling in Streptococcus pneumoniae by using seconeolitsine, a new topoisomerase I inhibitor. A homeostatic response allowing recovery of supercoiling was observed in cells treated with subinhibitory seconeolitsine concentrations. Supercoiling increases of 40.7% (6 μM) and 72.9% (8 μM) were lowered to 8.5% and 44.1%, respectively. Likewise, drug removal facilitated the recovery of cell viability and DNA-supercoiling. Transcription of topoisomerase I depended on the supercoiling level. Also specific binding of topoisomerase I to the gyrase A gene promoter was detected by chromatin-immunoprecipitation. The transcriptomic response to 8 μM seconeolitsine had two stages. An early stage, associated to an increase in supercoiling, affected 10% of the genome. A late stage, manifested by supercoiling recovery, affected 2% of the genome. Nearly 25% of the early responsive genes formed 12 clusters with a coordinated transcription. Clusters were 6.7-31.4 kb in length and included 9-22 responsive genes. These clusters partially overlapped with those observed under DNA relaxation, suggesting that bacteria manage supercoiling stress using pathways with common components. This is the first report of a coordinated global transcriptomic response that is triggered by an increase in DNA supercoiling in bacteria. | es_ES |
dc.description.sponsorship | Ministerio de Economía y Competitividad [BIO2014-55462-R to A.G.C.]. AJMG is the beholder of a Miguel Servet contract from the Spanish Ministry of Health. Funding for open access charge: Ministerio de Economía y Competitividad [BIO2014-55462-R to A.G.C.]. AJMG is the beholder of a Miguel Servet contract from the Spanish Ministry of Health. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject.mesh | Benzodioxoles | es_ES |
dc.subject.mesh | DNA | es_ES |
dc.subject.mesh | DNA Topoisomerases, Type I | es_ES |
dc.subject.mesh | DNA, Bacterial | es_ES |
dc.subject.mesh | DNA, Superhelical | es_ES |
dc.subject.mesh | Gene Expression Regulation, Bacterial | es_ES |
dc.subject.mesh | Genes, Bacterial | es_ES |
dc.subject.mesh | Homeostasis | es_ES |
dc.subject.mesh | Microbial Viability | es_ES |
dc.subject.mesh | Phenanthrenes | es_ES |
dc.subject.mesh | Streptococcus pneumoniae | es_ES |
dc.subject.mesh | Transcription, Genetic | es_ES |
dc.subject.mesh | Transcriptome | es_ES |
dc.subject.mesh | Multigene Family | es_ES |
dc.title | An increase in negative supercoiling in bacteria reveals topology-reacting gene clusters and a homeostatic response mediated by the DNA topoisomerase I gene | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial 4.0 International | * |
dc.identifier.pubmedID | 27378778 | es_ES |
dc.format.volume | 44 | es_ES |
dc.format.number | 15 | es_ES |
dc.format.page | 7292-303 | es_ES |
dc.identifier.doi | 10.1093/nar/gkw602 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1362-4962 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1093/nar/gkw602 | es_ES |
dc.identifier.journal | Nucleic acids research | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/BIO2014-55462-R to A.G.C | es_ES |
dc.rights.accessRights | open access | es_ES |