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dc.contributor.authorFerrandiz-Avellano, Maria-Jose 
dc.contributor.authorMartin-Galiano, Antonio Javier 
dc.contributor.authorArnanz, Cristina 
dc.contributor.authorCamacho-Soguero, Isabel
dc.contributor.authorTirado-Velez, JM 
dc.contributor.authorde la Campa, Adela G 
dc.date.accessioned2020-04-08T12:24:55Z
dc.date.available2020-04-08T12:24:55Z
dc.date.issued2016
dc.identifier.citationNucleic Acids Res. 2016 Sep 6;44(15):7292-303.es_ES
dc.identifier.issn0305-1048es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9487
dc.description.abstractWe studied the transcriptional response to an increase in DNA supercoiling in Streptococcus pneumoniae by using seconeolitsine, a new topoisomerase I inhibitor. A homeostatic response allowing recovery of supercoiling was observed in cells treated with subinhibitory seconeolitsine concentrations. Supercoiling increases of 40.7% (6 μM) and 72.9% (8 μM) were lowered to 8.5% and 44.1%, respectively. Likewise, drug removal facilitated the recovery of cell viability and DNA-supercoiling. Transcription of topoisomerase I depended on the supercoiling level. Also specific binding of topoisomerase I to the gyrase A gene promoter was detected by chromatin-immunoprecipitation. The transcriptomic response to 8 μM seconeolitsine had two stages. An early stage, associated to an increase in supercoiling, affected 10% of the genome. A late stage, manifested by supercoiling recovery, affected 2% of the genome. Nearly 25% of the early responsive genes formed 12 clusters with a coordinated transcription. Clusters were 6.7-31.4 kb in length and included 9-22 responsive genes. These clusters partially overlapped with those observed under DNA relaxation, suggesting that bacteria manage supercoiling stress using pathways with common components. This is the first report of a coordinated global transcriptomic response that is triggered by an increase in DNA supercoiling in bacteria.es_ES
dc.description.sponsorshipMinisterio de Economía y Competitividad [BIO2014-55462-R to A.G.C.]. AJMG is the beholder of a Miguel Servet contract from the Spanish Ministry of Health. Funding for open access charge: Ministerio de Economía y Competitividad [BIO2014-55462-R to A.G.C.]. AJMG is the beholder of a Miguel Servet contract from the Spanish Ministry of Health.es_ES
dc.language.isoenges_ES
dc.publisherOxford University Press es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subject.meshBenzodioxoles es_ES
dc.subject.meshDNA es_ES
dc.subject.meshDNA Topoisomerases, Type I es_ES
dc.subject.meshDNA, Bacterial es_ES
dc.subject.meshDNA, Superhelical es_ES
dc.subject.meshGene Expression Regulation, Bacterial es_ES
dc.subject.meshGenes, Bacterial es_ES
dc.subject.meshHomeostasis es_ES
dc.subject.meshMicrobial Viability es_ES
dc.subject.meshPhenanthrenes es_ES
dc.subject.meshStreptococcus pneumoniae es_ES
dc.subject.meshTranscription, Genetic es_ES
dc.subject.meshTranscriptome es_ES
dc.subject.meshMultigene Family es_ES
dc.titleAn increase in negative supercoiling in bacteria reveals topology-reacting gene clusters and a homeostatic response mediated by the DNA topoisomerase I genees_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial 4.0 International*
dc.identifier.pubmedID27378778es_ES
dc.format.volume44es_ES
dc.format.number15es_ES
dc.format.page7292-303es_ES
dc.identifier.doi10.1093/nar/gkw602es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1362-4962es_ES
dc.relation.publisherversionhttps://doi.org/10.1093/nar/gkw602es_ES
dc.identifier.journalNucleic acids researches_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/BIO2014-55462-R to A.G.Ces_ES
dc.rights.accessRightsopen accesses_ES


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Attribution-NonCommercial 4.0 International
Este Item está sujeto a una licencia Creative Commons: Attribution-NonCommercial 4.0 International