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dc.contributor.authorSolana, José Carlos 
dc.contributor.authorRamírez, Laura
dc.contributor.authorCook, Emma Cl
dc.contributor.authorHernández-García, Elena
dc.contributor.authorSacristán, Silvia
dc.contributor.authorMartín, M Elena
dc.contributor.authorManuel González, Víctor
dc.contributor.authorReguera, Rosa María
dc.contributor.authorBalaña-Fouce, Rafael
dc.contributor.authorFresno, Manuel
dc.contributor.authorRequena, José María
dc.contributor.authorIborra, Salvador 
dc.contributor.authorSoto, Manuel
dc.date.accessioned2020-04-07T07:40:37Z
dc.date.available2020-04-07T07:40:37Z
dc.date.issued2020-03-23
dc.identifier.citationVaccines (Basel). 2020 Mar 23;8(1). pii: E141.es_ES
dc.identifier.issn2076-393Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9448
dc.description.abstractLeishmania infantum parasites cause a severe form of visceral leishmaniasis in human and viscerocutaneous leishmaniasis in dogs. Recently, we reported that immunization with an attenuated L. infantum cell line, lacking the hsp70-II gene, protects against the development of murine cutaneous leishmaniasis. In this work, we analyzed the vaccine potential of this cell line towards the long-term protection against murine visceral leishmaniasis. This model shows an organ-dependent evolution of the disease. The infection can resolve in the liver but chronically affect spleen and bone marrow. Twelve weeks after subcutaneous administration of attenuated L. infantum, Bagg Albino (BALB/c) mice were challenged with infective L. infantum parasites expressing the luciferase-encoding gene. Combining in vivo bioimaging techniques with limiting dilution experiments, we report that, in the initial phase of the disease, vaccinated animals presented lower parasite loads than unvaccinated animals. A reduction of the severity of liver damage was also detected. Protection was associated with the induction of rapid parasite-specific IFN-γ production by CD4+ and CD8+ T cells. However, the vaccine was unable to control the chronic phase of the disease, since we did not find differences in the parasite burdens nor in the immune response at that time point.es_ES
dc.description.sponsorshipThe research made for this study was supported in Spain by grants from Ministerio de Ciencia e Innovación FISPI11/00095, SAF2017-83575-R and FISPI14/00366 (FEDER FUNDING) and the Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). S.I. is funded by RYC-2016-19463 and RTI2018-343 094484-B-I00 from Ministerio de Ciencia e Innovación. Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectIFN-γes_ES
dc.subjectT-cellses_ES
dc.subjectattenuated parasiteses_ES
dc.subjectmicees_ES
dc.subjectvaccinees_ES
dc.subjectvisceral leishmaniasises_ES
dc.titleSubcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Micees_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID32210040es_ES
dc.format.volume8es_ES
dc.format.number1es_ES
dc.format.page141es_ES
dc.identifier.doi10.3390/vaccines8010141es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderFundación Ramón Areceses_ES
dc.contributor.funderBanco Santanderes_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/vaccines8010141es_ES
dc.identifier.journalVaccineses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/FISPI11/00095es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/SAF2017-83575-Res_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/ FISPI14/00366es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/ISCIII-RETICRD16/0027/0008-FEDERes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/ RYC-2016-19463es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/RTI2018-343 094484-B-I00es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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