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dc.contributor.authorMartin-Vicente, Maria 
dc.contributor.authorGonzález-Riaño, Carolina
dc.contributor.authorBarbas, Coral
dc.contributor.authorJimenez-Sousa, Maria Angeles 
dc.contributor.authorBrochado-Kith, Oscar 
dc.contributor.authorResino, Salvador 
dc.contributor.authorMartinez, Isidoro 
dc.date.accessioned2020-04-03T07:38:13Z
dc.date.available2020-04-03T07:38:13Z
dc.date.issued2020
dc.identifier.citationPLoS One. 2020 Mar 26;15(3):e0230844.es_ES
dc.identifier.issn1932-6203es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9421
dc.description.abstractViral infections induce substantial metabolic changes in infected cells to optimize viral production while cells develop countermeasures to restrict that infection. Human respiratory syncytial virus (HRSV) is an infectious pathogen that causes severe lower respiratory tract infections (LRTI) in infants, the elderly, and immunocompromised adults for which no effective treatment or vaccine is currently available. In this study, variations in metabolite levels at different time points post-HRSV infection of epithelial cells were studied by untargeted metabolomics using liquid chromatography/mass spectrometry analysis of methanol cell extracts. Numerous metabolites were significantly upregulated after 18 hours post-infection, including nucleotides, amino acids, amino and nucleotide sugars, and metabolites of the central carbon pathway. In contrast, most lipid classes were downregulated. Additionally, increased levels of oxidized glutathione and polyamines were associated with oxidative stress in infected cells. These results show how HRSV infection influences cell metabolism to produce the energy and building blocks necessary for virus reproduction, suggesting potential therapeutic interventions against this virus.es_ES
dc.description.sponsorshipThis work was supported by the Instituto de Salud Carlos III, grant number PI15CIII/0024 to IM. URL: https://www.isciii.es/Paginas/Inicio.aspx The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleMetabolic changes during respiratory syncytial virus infection of epithelial cellses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID32214395es_ES
dc.format.volume15es_ES
dc.format.number3es_ES
dc.format.pagee0230844es_ES
dc.identifier.doi10.1371/journal.pone.0230844es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.description.peerreviewedNoes_ES
dc.identifier.e-issn1932-6203es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0230844es_ES
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI15CIII/0024es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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