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dc.contributor.authorXu, Wen
dc.contributor.authorLong, Lijiang
dc.contributor.authorZhao, Yuehui
dc.contributor.authorStevens, Lewis
dc.contributor.authorFelipe, Irene
dc.contributor.authorMunoz, Javier 
dc.contributor.authorEllis, Ronald E
dc.contributor.authorMcGrath, Patrick T
dc.identifier.citationElife. 2019 9;8es_ES
dc.description.abstractGenes can encode multiple isoforms, broadening their functions and providing a molecular substrate to evolve phenotypic diversity. Evolution of isoform function is a potential route to adapt to new environments. Here we show that de novo, beneficial alleles in the nurf-1 gene became fixed in two laboratory lineages of C. elegans after isolation from the wild in 1951, before methods of cryopreservation were developed. nurf-1 encodes an ortholog of BPTF, a large (>300 kD) multidomain subunit of the NURF chromatin remodeling complex. Using CRISPR-Cas9 genome editing and transgenic rescue, we demonstrate that in C. elegans, nurf-1 has split into two, largely non-overlapping isoforms (NURF-1.D and NURF-1.B, which we call Yin and Yang, respectively) that share only two of 26 exons. Both isoforms are essential for normal gametogenesis but have opposite effects on male/female gamete differentiation. Reproduction in hermaphrodites, which involves production of both sperm and oocytes, requires a balance of these opposing Yin and Yang isoforms. Transgenic rescue and genetic position of the fixed mutations suggest that different isoforms are modified in each laboratory strain. In a related clade of Caenorhabditis nematodes, the shared exons have duplicated, resulting in the split of the Yin and Yang isoforms into separate genes, each containing approximately 200 amino acids of duplicated sequence that has undergone accelerated protein evolution following the duplication. Associated with this duplication event is the loss of two additional nurf-1 transcripts, including the long-form transcript and a newly identified, highly expressed transcript encoded by the duplicated exons. We propose these lost transcripts are non-functional side products necessary to transcribe the Yin and Yang transcripts in the same cells. Our work demonstrates how gene sharing, through the production of multiple isoforms, can precede the creation of new, independent genes.es_ES
dc.description.sponsorshipNational Institute of General Medical Sciences R01GM114170 Patrick T McGrat National Institute of General Medical Sciences R01GM121688 Ronald E Ellis.The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.es_ES
dc.publishereLife Sciences Publications es_ES
dc.subjectC. eleganses_ES
dc.subjectIsoform evolutiones_ES
dc.subjectadaptive conflictes_ES
dc.subjectalternative transcriptses_ES
dc.subjectchromatin remodelinges_ES
dc.subjectevolutionary biologyes_ES
dc.subjectother Caenorhabditis specieses_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCaenorhabditis elegans es_ES
dc.subject.meshCaenorhabditis elegans Proteins es_ES
dc.subject.meshChromatin Assembly and Disassembly es_ES
dc.subject.meshChromosomal Proteins, Non-Histone es_ES
dc.subject.meshFemale es_ES
dc.subject.meshGametogenesis es_ES
dc.subject.meshMale es_ES
dc.subject.meshProtein Isoforms es_ES
dc.subject.meshEvolution, Moleculares_ES
dc.titleEvolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two geneses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderUnited States Department of Health & Human Services National Institutes of Health (NIH) - USA
dc.repisalud.orgCNIOCNIO::Unidades técnicas::Unidad de Proteómicaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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