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dc.contributor.authorde Oliveira, Haroldo Cesar 
dc.contributor.authorMonteiro, Maria Candida 
dc.contributor.authorRossi, Suelen Andreia 
dc.contributor.authorPemán, Javier
dc.contributor.authorRuiz-Gaitán, Alba
dc.contributor.authorMendes-Giannini, Maria José Soares
dc.contributor.authorMellado, Emilia 
dc.contributor.authorZaragoza, Oscar
dc.identifier.citationFront Cell Infect Microbiol. 2019 Apr 2;9:83.es_ES
dc.description.abstractCandida auris is an emerging fungal pathogen of great concern among the scientific community because it is causing an increasing number of hospital outbreaks of difficult management worldwide. In addition, isolates from this species frequently present reduced susceptibility to azole and echinocandin drugs. For this reason, it is necessary to develop new antifungal strategies to better control the disease caused by this yeast. In this work, we screened drugs from the Prestwick chemical library, which contains 1,280 off-patent compounds that are already approved by the Food and Drug Administration, with the aim of identifying molecules with antifungal activity against C. auris. In an initial screening, we looked for drugs that inhibited the growth of three different C. auris strains and found 27 of them which it did so. Ten active compounds were selected to test the susceptibility profile by using the EUCAST protocol. Antifungal activity was confirmed for seven drugs with MICs ranging from 0.5 to 64 mg/L. Some of these drugs were also tested in combination with voriconazole and anidulafungin at sub-inhibitory concentrations. Our results suggest synergistic interactions between suloctidil and voriconazole with fractional inhibitory concentration index (FICI) values of 0.11 to 0.5 and between ebselen and anidulafungin (FICI, 0.12 to 0.44). Our findings indicate that drug repurposing could be a viable alternative to managing infections by C. auris.es_ES
dc.description.sponsorshipOZ is funded by grants SAF2014-54336-R and SAF2017-86192-R1 from the Spanish Ministry for Economics, Industry and Competitivity. JP and AR-G are supported by Fondo de Investigación Sanitaria (FIS PI17_01538), EM is supported by Fondo de Investigación Sanitaria (FIS PI15_00019), EM and OZ were also sponsored by Plan Nacional de I+D+i 2013-2016 and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD16/CIII/0004/0003), co-financed by European Development Regional Fund ERDF A way to achieve Europe, Operative program Intelligent Growth 2014-2020. HdO was funded by postdoctoral fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP-BEPE 2016/20631-3). MM was funded by Fondo de Investigacion Sanitaria through a Sara Borrell Fellowship (grant CD13/00198). SR was supported by a fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPQ, program Ciências Sem Fronteiras (202436/2015-2).es_ES
dc.publisherFrontiers Media es_ES
dc.subjectCandida aurises_ES
dc.subjectDrug repurposinges_ES
dc.subject.meshAntifungal Agents es_ES
dc.subject.meshCandida es_ES
dc.subject.meshDrug Evaluation, Preclinical es_ES
dc.subject.meshDrug Repositioning es_ES
dc.subject.meshDrug Synergism es_ES
dc.subject.meshMicrobial Sensitivity Tests es_ES
dc.subject.meshSuloctidil es_ES
dc.subject.meshVoriconazole es_ES
dc.titleIdentification of Off-Patent Compounds That Present Antifungal Activity Against the Emerging Fungal Pathogen Candida aurises_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.identifier.journalFrontiers in cellular and infection microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/FIS PI17_01538es_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/FIS PI15_00019es_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/REIPI RD16/CIII/0004/0003es_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/FAPESP-BEPE 2016/20631-3es_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/grant CD13/00198es_ES
dc.rights.accessRightsopen accesses_ES

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