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dc.contributor.authorMartinez-Cruzado, Lucia
dc.contributor.authorTornin, Juan
dc.contributor.authorSantos, Laura
dc.contributor.authorRodriguez, Aida
dc.contributor.authorGarcia-Castro, Javier 
dc.contributor.authorMorís, Francisco
dc.contributor.authorRodriguez, Rene
dc.date.accessioned2020-02-28T07:59:05Z
dc.date.available2020-02-28T07:59:05Z
dc.date.issued2016
dc.identifier.citationSci Rep. 2016 Jun 13;6:27878.es_ES
dc.identifier.issn2045-2322es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9158
dc.description.abstractTumors evolve from initial tumorigenic events into increasingly aggressive behaviors in a process usually driven by subpopulations of cancer stem cells (CSCs). Mesenchymal stromal/stem cells (MSCs) may act as the cell-of-origin for sarcomas, and CSCs that present MSC features have been identified in sarcomas due to their ability to grow as self-renewed floating spheres (tumorspheres). Accordingly, we previously developed sarcoma models using human MSCs transformed with relevant oncogenic events. To study the evolution/emergence of CSC subpopulations during tumor progression, we compared the tumorigenic properties of bulk adherent cultures and tumorsphere-forming subpopulations both in the sarcoma cell-of-origin models (transformed MSCs) and in their corresponding tumor xenograft-derived cells. Tumor formation assays showed that the tumorsphere cultures from xenograft-derived cells, but not from the cell-of-origin models, were enriched in CSCs, providing evidence of the emergence of bona fide CSCs subpopulations during tumor progression. Relevant CSC-related factors, such as ALDH1 and SOX2, were increasingly upregulated in CSCs during tumor progression, and importantly, the increased levels and activity of ALDH1 in these subpopulations were associated with enhanced tumorigenicity. In addition to being a CSC marker, our findings indicate that ALDH1 could also be useful for tracking the malignant potential of CSC subpopulations during sarcoma evolution.es_ES
dc.description.sponsorshipThis work was supported by the Plan Nacional de I + D + i 2008-2011 [ISC III/FEDER (Miguel Servet Program CP11/00024) and RTICC (RD12/0036/0015)], the Plan Nacional de I + D + i 2013-2016 [MINECO/FEDER (SAF-2013-42946-R)] and the Plan de Ciencia Tecnología e Innovación del Principado de Asturias (GRUPIN14-003) to R.R; and the Plan Nacional de I + D + i 2008-2011 [ISC III/FEDER RTICC (RD12/0036/0027)] to J.G.-C.es_ES
dc.language.isoenges_ES
dc.publisherNature Researches_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAldehyde Dehydrogenase 1 es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCell Line, Tumor es_ES
dc.subject.meshHumans es_ES
dc.subject.meshIsoenzymes es_ES
dc.subject.meshLung Neoplasms es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred NOD es_ES
dc.subject.meshMice, SCID es_ES
dc.subject.meshNeoplastic Stem Cells es_ES
dc.subject.meshRNA Interference es_ES
dc.subject.meshRNA, Small Interfering es_ES
dc.subject.meshRetinal Dehydrogenase es_ES
dc.subject.meshSOXB1 Transcription Factors es_ES
dc.subject.meshSarcoma es_ES
dc.subject.meshTime-Lapse Imaging es_ES
dc.subject.meshTransplantation, Heterologous es_ES
dc.titleAldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populationses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID27292183es_ES
dc.format.volume6es_ES
dc.format.number1es_ES
dc.format.page27878es_ES
dc.identifier.doi10.1038/srep27878es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderGobierno del Principado de Asturiases_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn2045-2322es_ES
dc.relation.publisherversionhttps://doi.org/10.1038/srep27878es_ES
dc.identifier.journalScientific reportses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CP11/00024es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0036/0015es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF-2013-42946-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/GRUPIN14-003es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0036/0027es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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