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dc.contributor.authorVazquez-Moron, Sonia 
dc.contributor.authorRyan, Pablo
dc.contributor.authorArdizone Jimenez, Beatriz 
dc.contributor.authorMartín, Dolores
dc.contributor.authorTroya, Jesus
dc.contributor.authorCuevas, Guillermo
dc.contributor.authorValencia, Jorge
dc.contributor.authorJimenez-Sousa, Maria Angeles 
dc.contributor.authorAvellón, Ana 
dc.contributor.authorResino, Salvador
dc.identifier.citationSci Rep. 2018 Jan 30;8(1):1858.es_ES
dc.description.abstractBoth hepatitis C virus (HCV) infection and human immunodeficiency virus (HIV) infection are underdiagnosed, particularly in low-income countries and in difficult-to-access populations. Our aim was to develop and evaluate a methodology for the detection of HCV and HIV infection based on capillary dry blood spot (DBS) samples taken under real-world conditions. We carried out a cross-sectional study of 139 individuals (31 healthy controls, 68 HCV-monoinfected patients, and 40 HCV/HIV-coinfected patients). ELISA was used for anti-HCV and anti-HIV antibody detection; and SYBR Green RT-PCR was used for HCV-RNA detection. The HIV serological analysis revealed 100% sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). The HCV serological analysis revealed a sensitivity of 92.6%, specificity of 100%, PPV of 100%, and NPV of 79.5%. Finally, the HCV-RNA detection test revealed a detection limit of 5 copies/µl with an efficiency of 100% and sensitivity of 99.1%, specificity of 100%, PPV of 100%, and NPV of 96.9%. In conclusion, our methodology was able to detect both HCV infection and HIV infection from the same DBS sample with good diagnostic performance. Screening for HCV and HIV using DBS might be a key strategy in the implementation of national programs for the control of both infections.es_ES
dc.description.sponsorshipWe acknowledge the patients’ involvement in this study. The authors thank Thomas O’Boyle for writing assistance during the preparation of the manuscript. This study was supported by grants from Fondo de Investigación de Sanidad en España (FIS) [Spanish Health Founds for Research] [grant numbers PI14CIII/00011] Red Española de Investigación en SIDA (RIS) [AIDS Research Network] [grant numbers RD16CIII/0002/0002RD16] and a research grant from Merck Sharpe & Dohme (MISP IIS#54846).es_ES
dc.publisherNature Publishing Group es_ES
dc.subject.meshAdult es_ES
dc.subject.meshCross-Sectional Studies es_ES
dc.subject.meshDried Blood Spot Testing es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHIV es_ES
dc.subject.meshHIV Infections es_ES
dc.subject.meshHepacivirus es_ES
dc.subject.meshHepatitis C es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMale es_ES
dc.subject.meshMiddle Aged es_ES
dc.subject.meshSpecimen Handling es_ES
dc.subject.meshPoint-of-Care Systems es_ES
dc.titleEvaluation of dried blood spot samples for screening of hepatitis C and human immunodeficiency virus in a real-world settinges_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderRed de Investigación Cooperativa en Investigación en Sida (España) 
dc.identifier.journalScientific reportses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MISP IIS#54846es_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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