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dc.contributor.authorDomenech, Arnau
dc.contributor.authorMoreno, Javier 
dc.contributor.authorArdanuy, Carmen
dc.contributor.authorLiñares, Josefina
dc.contributor.authorde la Campa, Adela G 
dc.contributor.authorMartin-Galiano, Antonio Javier 
dc.date.accessioned2020-02-21T10:57:51Z
dc.date.available2020-02-21T10:57:51Z
dc.date.issued2016
dc.identifier.citationFront Microbiol. 2016 Mar 31;7:420.es_ES
dc.identifier.issn1664-302Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9130
dc.description.abstractThe diverse pneumococcal diseases are associated with different pneumococcal lineages, or clonal complexes. Nevertheless, intra-clonal genomic variability, which influences pathogenicity, has been reported for surface virulence factors. These factors constitute the communication interface between the pathogen and its host and their corresponding genes are subjected to strong selective pressures affecting functionality and immunogenicity. First, the presence and allelic dispersion of 97 outer protein families were screened in 19 complete pneumococcal genomes. Seventeen families were deemed variable and were then examined in 216 draft genomes. This procedure allowed the generation of binary vectors with 17 positions and the classification of strains into surfotypes. They represent the outer protein subsets with the highest inter-strain discriminative power. A total of 116 non-redundant surfotypes were identified. Those sharing a critical number of common protein features were hierarchically clustered into 18 surfogroups. Most clonal complexes with comparable epidemiological characteristics belonged to the same or similar surfogroups. However, the very large CC156 clonal complex was dispersed over several surfogroups. In order to establish a relationship between surfogroup and pathogenicity, the surfotypes of 95 clinical isolates with different serogroup/serotype combinations were analyzed. We found a significant correlation between surfogroup and type of pathogenic behavior (primary invasive, opportunistic invasive, and non-invasive). We conclude that the virulent behavior of S. pneumoniae is related to the activity of collections of, rather than individual, surface virulence factors. Since surfotypes evolve faster than MLSTs and directly reflect virulence potential, this novel typing protocol is appropriate for the identification of emerging clones.es_ES
dc.description.sponsorshipThis work was supported by a Miguel Servet contract from the Spanish Ministry of Health to AM, Plan Nacional de I+D+I of the Ministry of Science and Innovation (BIO2011-25343, BIO2014-555462-R, SAF2012-39444-C02), Fondo de Investigaciones Sanitarias de la Seguridad Social (PI11/00763) and Fondo Europeo de Desarrollo Regional (FEDER). CIBER Enfermedades Respiratorias is an initiative of the Instituto de Salud Carlos III.es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectdiagnosises_ES
dc.subjectemergent cloneses_ES
dc.subjectgenomicses_ES
dc.subjectsurface proteinses_ES
dc.subjectvirulence factorses_ES
dc.titleA Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteinses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID27064593es_ES
dc.format.volume7es_ES
dc.format.page420es_ES
dc.identifier.doi10.3389/fmicb.2016.00420es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2016.00420es_ES
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2011-25343es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/ BIO2014-555462-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/F2012-39444-C02es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI11/00763es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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