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dc.contributor.authorRossi, Suelen Andreia 
dc.contributor.authorTrevijano-Contador, Nuria 
dc.contributor.authorScorzoni, Liliana 
dc.contributor.authorMesa-Arango, Ana Cecilia 
dc.contributor.authorde Oliveira, Haroldo Cesar
dc.contributor.authorWerther, Karin
dc.contributor.authorde Freitas Raso, Tânia
dc.contributor.authorMendes-Giannini, Maria J S
dc.contributor.authorZaragoza, Oscar 
dc.contributor.authorFusco-Almeida, Ana M
dc.date.accessioned2020-02-21T10:57:32Z
dc.date.available2020-02-21T10:57:32Z
dc.date.issued2016
dc.identifier.citationFront Microbiol. 2016 Feb 16;7:153.es_ES
dc.identifier.issn1664-302Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9128
dc.description.abstractCryptococcus sp. are responsible for around 1 million cases of meningitis every year. Fluconazole (FLU) is commonly used in the treatment of cryptococcosis, mainly in immunocompromised patients and the resistance is usually reported after long periods of treatment. In this study, the morphological characterization and virulence profile of FLU-susceptible and FLU-resistant clinical and environmental isolates of C. neoformans and C. gattii were performed both in vitro and in vivo using the Galleria mellonella model. FLU-susceptible isolates from C. neoformans were significantly more virulent than the FLU-resistant isolates. FLU-susceptible C. gattii isolates showed a different virulence profile from C. neoformans isolates where only the environmental isolate, CL, was more virulent compared with the resistant isolates. Cell morphology and capsule size were analyzed and the FLU-resistant isolates did not change significantly compared with the most sensitive isolates. Growth at 37°C was also evaluated and in both species, the resistant isolates showed a reduced growth at this temperature, indicating that FLU resistance can affect their growth. Based on the results obtained is possible suggest that FLU resistance can influence the morphology of the isolates and consequently changed the virulence profiles. The most evident results were observed for C. neoformans showing that the adaptation of isolates to antifungal selective pressure influenced the loss of virulence.es_ES
dc.description.sponsorshipThis work was supported by the Programa Institucional de Doutorado Sanduíche no Exterior from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PDSE-CAPES – 462 1909-13-4), Rede Nacional de Métodos Alternativos – Conselho Nacional Científico e Tecnológico (RENAMA-CNPq 403586/2012-7) and Programa de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da Unesp (PADC/FCF). OZ is funded by grant SAF2011-25140 by the Spanish Ministry for Economics and Competitivity.es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Media es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCryptococcus sp.es_ES
dc.subjectGalleria mellonellaes_ES
dc.subjectFluconazolees_ES
dc.subjectResistancees_ES
dc.subjectVirulencees_ES
dc.titleImpact of Resistance to Fluconazole on Virulence and Morphological Aspects of Cryptococcus neoformans and Cryptococcus gattii Isolateses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID26909069es_ES
dc.format.volume7es_ES
dc.format.page153es_ES
dc.identifier.doi10.3389/fmicb.2016.00153es_ES
dc.contributor.funderNational Council for Scientific and Technological Development (Brasil) 
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2016.00153es_ES
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PDSE-CAPES – 462 1909-13-4es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RENAMA-CNPq 403586/2012-7es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2011-25140es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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