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dc.contributor.author | Rossi, Suelen Andreia | |
dc.contributor.author | Trevijano-Contador, Nuria | |
dc.contributor.author | Scorzoni, Liliana | |
dc.contributor.author | Mesa-Arango, Ana Cecilia | |
dc.contributor.author | de Oliveira, Haroldo Cesar | |
dc.contributor.author | Werther, Karin | |
dc.contributor.author | de Freitas Raso, Tânia | |
dc.contributor.author | Mendes-Giannini, Maria J S | |
dc.contributor.author | Zaragoza, Oscar | |
dc.contributor.author | Fusco-Almeida, Ana M | |
dc.date.accessioned | 2020-02-21T10:57:32Z | |
dc.date.available | 2020-02-21T10:57:32Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Front Microbiol. 2016 Feb 16;7:153. | es_ES |
dc.identifier.issn | 1664-302X | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/9128 | |
dc.description.abstract | Cryptococcus sp. are responsible for around 1 million cases of meningitis every year. Fluconazole (FLU) is commonly used in the treatment of cryptococcosis, mainly in immunocompromised patients and the resistance is usually reported after long periods of treatment. In this study, the morphological characterization and virulence profile of FLU-susceptible and FLU-resistant clinical and environmental isolates of C. neoformans and C. gattii were performed both in vitro and in vivo using the Galleria mellonella model. FLU-susceptible isolates from C. neoformans were significantly more virulent than the FLU-resistant isolates. FLU-susceptible C. gattii isolates showed a different virulence profile from C. neoformans isolates where only the environmental isolate, CL, was more virulent compared with the resistant isolates. Cell morphology and capsule size were analyzed and the FLU-resistant isolates did not change significantly compared with the most sensitive isolates. Growth at 37°C was also evaluated and in both species, the resistant isolates showed a reduced growth at this temperature, indicating that FLU resistance can affect their growth. Based on the results obtained is possible suggest that FLU resistance can influence the morphology of the isolates and consequently changed the virulence profiles. The most evident results were observed for C. neoformans showing that the adaptation of isolates to antifungal selective pressure influenced the loss of virulence. | es_ES |
dc.description.sponsorship | This work was supported by the Programa Institucional de Doutorado Sanduíche no Exterior from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PDSE-CAPES – 462 1909-13-4), Rede Nacional de Métodos Alternativos – Conselho Nacional Científico e Tecnológico (RENAMA-CNPq 403586/2012-7) and Programa de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da Unesp (PADC/FCF). OZ is funded by grant SAF2011-25140 by the Spanish Ministry for Economics and Competitivity. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Cryptococcus sp. | es_ES |
dc.subject | Galleria mellonella | es_ES |
dc.subject | Fluconazole | es_ES |
dc.subject | Resistance | es_ES |
dc.subject | Virulence | es_ES |
dc.title | Impact of Resistance to Fluconazole on Virulence and Morphological Aspects of Cryptococcus neoformans and Cryptococcus gattii Isolates | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 26909069 | es_ES |
dc.format.volume | 7 | es_ES |
dc.format.page | 153 | es_ES |
dc.identifier.doi | 10.3389/fmicb.2016.00153 | es_ES |
dc.contributor.funder | National Council for Scientific and Technological Development (Brasil) | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.relation.publisherversion | https://doi.org/10.3389/fmicb.2016.00153 | es_ES |
dc.identifier.journal | Frontiers in microbiology | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PDSE-CAPES – 462 1909-13-4 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RENAMA-CNPq 403586/2012-7 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2011-25140 | es_ES |
dc.rights.accessRights | open access | es_ES |