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dc.contributor.authorFerrandiz-Avellano, Maria-Jose 
dc.contributor.authorCarreño, David 
dc.contributor.authorAyora, Silvia
dc.contributor.authorde la Campa, Adela G 
dc.date.accessioned2020-02-21T10:56:36Z
dc.date.available2020-02-21T10:56:36Z
dc.date.issued2018
dc.identifier.citationFront Microbiol. 2018 Mar 19;9:493.es_ES
dc.identifier.issn1664-302Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9123
dc.description.abstractThe histone-like protein HU is a conserved nucleoid-associated protein that is involved in the maintenance of the bacterial chromosome architecture. It is the only known nucleoid-associated protein in Streptococcus pneumoniae, but it has not been studied. The pneumococcal gene encoding this protein, hlp, is shown herein to be essential for cell viability. Its disruption was only possible either when it was duplicated in the chromosome and its expression induced from the P Zn promoter, or when hlp was cloned into a plasmid under the control of the inducible P mal promoter. In vitro assays indicated that pneumococcal HU shows a preference for binding to supercoiled DNA rather than to linear or nicked DNA. In vivo experiments in which the amount of HU was manipulated showed a relationship between the amount of HU and the level of DNA supercoiling. A twofold reduction in the amount of HU triggered a 21% increase in DNA relaxation in untreated cells. However, in cells treated with novobiocin, a drug that relaxes DNA by inhibiting DNA gyrase, a 35% increase in DNA relaxation was observed, instead of the expected 20% in cells with a constitutive HU amount. Conversely, a fourfold HU increase caused only 14% of DNA relaxation in the presence of novobiocin. Taken together, these results support an essential role for HU in the maintenance of DNA supercoiling in S. pneumoniae.es_ES
dc.description.sponsorshipThis work was supported by the Ministerio de Economía y Competitividad (BIO2014-55462-R and BIO2017-82951-R).es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Media es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectHUes_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.subjectHistone-like proteines_ES
dc.subjectNucleoides_ES
dc.subjectSupercoilinges_ES
dc.titleHU of Streptococcus pneumoniae Is Essential for the Preservation of DNA Supercoilinges_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID29662473es_ES
dc.format.volume9es_ES
dc.format.page493es_ES
dc.identifier.doi10.3389/fmicb.2018.00493es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2018.00493es_ES
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2014-55462-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2017-82951-Res_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional