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dc.contributor.authorVillalon-Panzano, Pilar 
dc.contributor.authorOrtega, Montserrat 
dc.contributor.authorSaez-Nieto, Juan A 
dc.contributor.authorCarrasco, Gema 
dc.contributor.authorMedina-Pascual, Maria Jose 
dc.contributor.authorGarrido, Noelia 
dc.contributor.authorValdezate, Sylvia 
dc.date.accessioned2020-02-21T10:56:06Z
dc.date.available2020-02-21T10:56:06Z
dc.date.issued2019
dc.identifier.citationFront Microbiol. 2019 Mar 22;10:593.es_ES
dc.identifier.issn1664-302Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9120
dc.description.abstractOur objective was to improve current knowledge of sporadic (Spo) nosocomial Acinetobactercalcoaceticus-Acinetobacter baumannii (Acb) complex populations, and thus better understand the epidemiology of Spo and endemoepidemic (EE) strains. Between 1999 and 2010, 133 isolates of Spo Acb complex were obtained from a single hospital. Species were identified by gyrB-PCR, and via gyrB- and rpoB-sequencing. Clonal analysis was undertaken using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. Susceptibility to antimicrobial agents was determined by microdilution and E-tests. Carbapenemase genes were detected by PCR. One hundred and one PFGE types were detected. A. baumannii was the most common (67/101 PFGE types), followed by Acinetobacter pittii (22/101), Acinetobacter lactucae (6/101), and Acinetobacter calcoaceticus (2/101). gyrB, rpoB1, and rpoB2 sequencing returned 49, 13, and 16 novel sequences, respectively. Sixty-three sequence types (STs) (38 new STs and 66 new alleles) were detected; the most common were ST2 (29/133 isolates) and ST132 (14/133). Twenty-six OXA-51 allelic variants were detected, nine of which were novel. The PFGE types were generally susceptible (88/101) to all the tested antimicrobials; 3/101 were carbapenem-resistant due to the presence of the genetic structure ISAba2-blaOXA-58-like-ISAba3, and 2/101 were multidrug-resistant. It can be concluded that the examined Spo Acb complex population was mainly composed of A. baumannii. Many different clones were detected (with ST2 clearly dominant), all largely susceptible to antimicrobials; multidrug resistance was rare. In contrast, a previously examined EE Acb population was composed of just four expanding, multidrug-resistant A. baumannii clones -ST2, ST3, ST15, and ST80-.es_ES
dc.description.sponsorshipWe thank Dr. Teresa Cabezas for the isolation and submission of the Acinetobacter spp. strains to the Reference and Research Laboratory for Taxonomy at the CNM, and Adrian Burton for editing and language assistance (Physical Evidence Scientific Translations; http://physicalevidence.es/english/welcome). This publication made use of the Acinetobacter baumannii MLST website (https://pubmlst.org/abaumannii/) hosted by the University of Oxford (Jolley and Maiden 2010, BMC Bioinformatics, 11:595), the development of which was funded by the Wellcome Trust.es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAcinetobacter calcoaceticus-baumannii complexes_ES
dc.subjectantimicrobial susceptibilityes_ES
dc.subjectclonal distributiones_ES
dc.subjectepidemic strainses_ES
dc.subjectspecies identificationes_ES
dc.subjectsporadic strainses_ES
dc.titleDynamics of a Sporadic Nosocomial Acinetobacter calcoaceticus - Acinetobacter baumannii Complex Populationes_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID30967856es_ES
dc.format.volume10es_ES
dc.format.page593es_ES
dc.identifier.doi10.3389/fmicb.2019.00593es_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2019.00593es_ES
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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