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dc.contributor.authorRivero-Menendez, Olga 
dc.contributor.authorNavarro-Rodriguez, Patricia
dc.contributor.authorBernal-Martinez, Leticia 
dc.contributor.authorMartin-Cano, Gema 
dc.contributor.authorLopez-Perez, Laura 
dc.contributor.authorSanchez-Romero, Isabel
dc.contributor.authorPerez-Ayala, Ana
dc.contributor.authorCapilla, Javier
dc.contributor.authorZaragoza, Oscar 
dc.contributor.authorAlastruey-Izquierdo, Ana
dc.identifier.citationFront Microbiol. 2019 Jul 11;10:1585.es_ES
dc.description.abstractThe pathogenic yeast Candida glabrata has become a public health issue due to the increasing number of echinocandin resistant clinical strains reported. In this study, acquisition and development of resistance to this antifungal class were studied in serial C. glabrata isolates from five patients admitted in two Spanish hospitals with a resistant profile against echinocandins associated with different mutations in hot-spot 1 of FKS2 gene. For two of these patients susceptible FKS wild-type isolates obtained prior to resistant ones were also investigated. Isolates were genotyped using multilocus sequence typing and microsatellite length polymorphism techniques, which yielded comparable results. Susceptible and resistant isolates from the same patient had the same genotype, being sequence type (ST) 3 the most prevalent among them. Isolates with different FKS mutations but the same ST were present in the same patient. MSH2 gene alterations were also studied to investigate their correlation with antifungal resistance acquisition but no association was found with antifungal resistance nor with specific genotypes. In vitro exposure to increasing concentrations of micafungin to susceptible isolates developed colonies carrying FKS mutations in agar plates containing a minimum concentration of 0.06 mg/L of micafungin after less than 48 h of exposure. We investigated the correlation between development of resistance and genotype in a set of susceptible strains after being in vitro exposed to micafungin and anidulafungin but no correlation was found. Mutant prevention concentration values and spontaneous growth frequencies after selection with both echinocandins were statistically similar, although FKS mutant colonies were more abundant after micafungin exposure (p < 0.001). Mutation S663P and F659 deletion were the most common ones found after selection with both echinocandins.es_ES
dc.description.sponsorshipThis work was supported by the Fondo de Investigación Sanitaria (Grant FI14CIII/00025 to OR-M and research projects PI13/02145 and PI16CIII/00035 to AA-I), and also supported by the Plan Nacional de I+D+i 2013–2016 and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Ciencia, Innovación y Universidades, Spanish Network for Research in Infectious Diseases (REIPI RD16CIII/0004/0003) – co-financed by the European Development Regional Fund “A way to achieve Europe,” Operative Program Intelligent Growth 2014–2020.es_ES
dc.publisherFrontiers Media es_ES
dc.subjectCandida glabrataes_ES
dc.subjectantifungal resistancees_ES
dc.titleClinical and Laboratory Development of Echinocandin Resistance in Candida glabrata: Molecular Characterizationes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) 
dc.contributor.funderRETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España) 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/REIPI RD16CIII/0004/0003es_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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