Show simple item record

dc.contributor.authorLuque, Daniel 
dc.contributor.authorMata, Carlos P
dc.contributor.authorSuzuki, Nobuhiro
dc.contributor.authorGhabrial, Said A
dc.contributor.authorCastón, José R
dc.date.accessioned2020-01-29T11:47:21Z
dc.date.available2020-01-29T11:47:21Z
dc.date.issued2018
dc.identifier.citationViruses. 2018 Sep 7;10(9). pii: E481.es_ES
dc.identifier.issn1999-4915es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8972
dc.description.abstractMost fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. The atomic structure of the T = 1 capsids of four mycoviruses was resolved: the L-A virus of Saccharomyces cerevisiae (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). These capsids show structural variations of the same framework, with 60 asymmetric or symmetric homodimers for ScV-L-A and PsV-F, respectively, monomers with a duplicated similar domain for PcV, and heterodimers of two different proteins for RnQV1. Mycovirus capsid proteins (CP) share a conserved α-helical domain, although the latter may carry different peptides inserted at preferential hotspots. Insertions in the CP outer surface are likely associated with enzymatic activities. Within the capsid, fungal dsRNA viruses show a low degree of genome compaction compared to reoviruses, and contain one to two copies of the RNA-polymerase complex per virion.es_ES
dc.description.sponsorshipThis work was supported by grants from the Spanish Ministry of Economy and Competitivity (BFU2017-88736-R to J.R.C), and the Comunidad Autónoma de Madrid (S2013/MIT-2807 to J.R.C), and Grants-in-Aid for Scientific Research on Innovative Areas from the Japanese Ministry of Education, Culture, Sports, Science and Technology (KAKENHI 25252011 and 16H06436, 16H06429 and 16K21723 to N.S).es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPcVes_ES
dc.subjectPsV-Fes_ES
dc.subjectRnQV1es_ES
dc.subjectScV-L-Aes_ES
dc.subjectcapsid proteines_ES
dc.subjectcapsid structurees_ES
dc.subjectdsRNA viruses_ES
dc.subjectmycoviruses_ES
dc.subjectviral lineagees_ES
dc.subjectvirus evolutiones_ES
dc.subject.meshCapsid es_ES
dc.subject.meshCapsid Proteins es_ES
dc.subject.meshFungal Viruses es_ES
dc.subject.meshPenicillium chrysogenum es_ES
dc.subject.meshProtein Conformation es_ES
dc.subject.meshRNA Viruses es_ES
dc.subject.meshSaccharomyces cerevisiae es_ES
dc.subject.meshXylariales es_ES
dc.titleCapsid Structure of dsRNA Fungal Viruseses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID30205532es_ES
dc.format.volume10es_ES
dc.format.number9es_ES
dc.format.page481es_ES
dc.identifier.doi10.3390/v10090481es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderMinistry of Education, Culture, Sports, Science and Technology (Japan)es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1999-4915es_ES
dc.relation.publisherversionhttps://doi.org/10.3390/v10090481es_ES
dc.identifier.journalViruseses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BFU2017-88736-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/S2013/MIT-2807es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/KAKENHI 25252011es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/16H06436es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/16H06429es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/16K21723es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


Files in this item

Acceso Abierto
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Atribución 4.0 Internacional
This item is licensed under a: Atribución 4.0 Internacional