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dc.contributor.authorLorenzo-Redondo, Ramon 
dc.contributor.authorBorderia, Antonio V. 
dc.contributor.authorLopez-Galindez, Luis Cecilio
dc.identifier.citationJ Virol. 2011 Feb;85(4):1861-70. doi: 10.1128/JVI.01254-10. Epub 2010 Nov 24.es_ES
dc.description.abstractThe study on the evolutionary consequences of an RNA viral population's fluctuations can be approached by in vitro experiments. This work describes the fitness recovery of HIV-1 after 20 large-population passages in 10 debilitated clones. The serial passages promoted an increase in viral fitness. In addition, we detected a significant number of mutations fixed in the complete genome consensus sequence of the final viral populations. Among the mutations, events of convergent evolution with important phenotypic characteristics occurred in several independent clones. One common change, V35I, in the nuclear localization signal of the p17 protein appeared in four viruses of three different lineages. Other common alterations mapped in position E196K of the reverse transcriptase or in position S316K of the V3 loop of the gp120 residue that is associated with the X4/R5 phenotype. Together with this mutational analysis, we studied the quasispecies heterogeneity of the initial and final viruses, revealing that fitness increase correlated with an augmentation in the genetic heterogeneity of viral quasispecies. However, while heterogeneity was mostly composed of synonymous (dS) mutations in the first 10 passages performed, at passage 21 it switched to nonsynonymous (dN) substitutions, with significant differences in dN - dS values between passages 11 and 21. In summary, the HIV-1 in vitro fitness recovery depicts a multiphase process occurring first by generation of mutations followed by fixation of the beneficial ones, depicting a classical Darwinian process.es_ES
dc.description.sponsorshipWork at CNM is supported by grant SAF 2007-61036 of the Ministerio de Ciencia e Innovación, by Fundacion para la Investigacion y Prevencion del SIDA en España (FIPSE) grants 36558/06, 36641/07, 36779/08, and 360766/09, and in part by the Red Temática Cooperativa de Investigación en SIDA (Red de grupos 173) of the Fondo de Investigaciones Sanitarias de la Seguridad Social (FISss).es_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.relationinfo:eu-repo/grantAgreement/ES/SAF 2007-61036es_ES
dc.subject.meshHIV Antigens es_ES
dc.subject.meshHIV Envelope Protein gp120 es_ES
dc.subject.meshHIV Reverse Transcriptase es_ES
dc.subject.meshHIV-1 es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMolecular Sequence Data es_ES
dc.subject.meshPhenotype es_ES
dc.subject.meshRNA, Viral es_ES
dc.subject.meshSequence Analysis, DNA es_ES
dc.subject.meshSerial Passage es_ES
dc.subject.meshgag Gene Products, Human Immunodeficiency Virus es_ES
dc.subject.meshEvolution, Moleculares_ES
dc.subject.meshGenome, Viral es_ES
dc.subject.meshMutation es_ES
dc.titleCONSULTACOPY Dynamics of in vitro fitness recovery of HIV-1es_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderFundación para la Investigación y la Prevención del Sida en Españaes_ES
dc.contributor.funderRed Temática Cooperativa de Investigación en Sida (España)es_ES
dc.identifier.journalJournal of virologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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